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dc.contributor.authorBushell, Simon R.
dc.contributor.authorLou, Hubing
dc.contributor.authorWallat, Gregor D.
dc.contributor.authorBeis, Konstantinos
dc.contributor.authorWhitfield, Chris
dc.contributor.authorNaismith, James H.
dc.identifier.citationBushell , S R , Lou , H , Wallat , G D , Beis , K , Whitfield , C & Naismith , J H 2010 , ' Crystallization and preliminary diffraction analysis of Wzi, a member of the capsule export and assembly pathway in Escherichia coli ' , Acta Crystallographica. Section F, Structural biology and crystallization communications , vol. 66 , no. 12 , pp. 1621-1625 .
dc.identifier.otherPURE: 5569882
dc.identifier.otherPURE UUID: f7bd54f6-a3c2-4520-b37c-113eade9f3cd
dc.identifier.otherWOS: 000285064800018
dc.identifier.otherScopus: 78650146359
dc.descriptionSupported by a grant from The Wellcome Trust (081862/Z/06/Z)en
dc.description.abstractExternal polysaccharide capsules provide a physical barrier that is employed by many species of bacteria for the purposes of host evasion and persistence. Wzi is a 53 kDa outer membrane beta-barrel protein that is thought to play a role in the attachment of group 1 capsular polysaccharides to the cell surface. The purification and crystallization of an Escherichia coli homologue of Wzi is reported and diffraction data from native and selenomethionine-incorporated protein crystals are presented. Crystals of C-terminally His(6)-tagged Wzi diffracted to 2.8 A resolution. Data processing showed that the crystals belonged to the orthorhombic space group C222, with unit-cell parameters a = 128.8, b = 152.8, c = 94.4 A, alpha = beta = gamma = 90 degrees. A His-tagged selenomethionine-containing variant of Wzi has also been crystallized in the same space group and diffraction data have been recorded to 3.8 A resolution. Data processing shows that the variant crystal has similar unit-cell parameters to the native crystal.
dc.relation.ispartofActa Crystallographica. Section F, Structural biology and crystallization communicationsen
dc.rightsThis is an open access article distributed under a Creative Commons licence ( which permits you to reuse and copy the article provided the original authors and source are cited.en
dc.subjectPolysaccharide capsulesen
dc.subjectEscherichia colien
dc.subjectColanic aciden
dc.subjectQD Chemistryen
dc.titleCrystallization and preliminary diffraction analysis of Wzi, a member of the capsule export and assembly pathway in Escherichia colien
dc.typeJournal articleen
dc.contributor.sponsorThe Wellcome Trusten
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Chemistryen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews. EaSTCHEMen
dc.description.statusPeer revieweden

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