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dc.contributor.authorSogabe, Shunsuke
dc.contributor.authorNakanishi, Nagayasu
dc.contributor.authorDegnan, Bernard
dc.date.accessioned2019-06-18T11:30:06Z
dc.date.available2019-06-18T11:30:06Z
dc.date.issued2016-03-08
dc.identifier.citationSogabe , S , Nakanishi , N & Degnan , B 2016 , ' The ontogeny of choanocyte chambers during metamorphosis in the demosponge Amphimedon queenslandica ' , EvoDevo , vol. 7 , 6 . https://doi.org/10.1186/s13227-016-0042-xen
dc.identifier.otherPURE: 259341021
dc.identifier.otherPURE UUID: 6be0847c-969d-4bc4-a48e-5a77909950c3
dc.identifier.otherScopus: 85007309119
dc.identifier.urihttp://hdl.handle.net/10023/17917
dc.description.abstractBackground The aquiferous body plan of poriferans revolves around internal chambers comprised of choanocytes, a cell type structurally similar to choanoflagellates. These choanocyte chambers perform a range of physiological and developmental functions, including the capture of food and the generation of stem cells. Despite the increasing interest for choanocytes as sponge stem cells, there is limited knowledge on the development of choanocyte chambers. Using a combination of cell lineage tracing, antibody staining and EdU labeling, here we examine the development of choanocytes and the chambers they comprise during metamorphosis in the marine demosponge Amphimedon queenslandica. Results Lineage-tracing experiments show that larval epithelial cells transform into mesenchymal pluripotent stem cells, resembling archeocytes, within 24 h of initiating metamorphosis. By 36 h, some of these labeled archeocyte-like cells have differentiated into choanocytes that will form the first postlarval choanocyte chambers. Non-labeled cells also contribute to these primary choanocyte chambers, consistent with these chambers being a chimera of multiple transdifferentiated larval cell types and not the proliferation of a single choanocyte precursor. Moreover, cell proliferation assays demonstrate that, following the initial formation of choanocyte chambers, chambers grow at least partially by the proliferation of choanocytes within the chamber, although recruitment of individual cells into established chambers also appears to occur. EdU labeling of postlarvae and juveniles reveals that choanocyte chambers are the primary location of cell proliferation during metamorphosis. Conclusion Our results show that multiple larval cell lineages typically contribute to formation of individual choanocyte chambers at metamorphosis, contrary to previous reports in other species that show sponge choanocyte chambers form clonally. Choanocytes in postlarval and juvenile A. queenslandica chambers can also divide, with choanocyte chambers being the primary location of cell proliferation. Interestingly, the level of cell proliferation varies greatly between chambers and appears to be contingent on the size, location and developmental state of the chamber. Small chambers on the periphery of the body tend to possess more dividing cells. As choanocytes can also dedifferentiate into archeocyte-like cells, cell proliferation in chambers may not only contribute to chamber growth and self-renewal but also increase the number of pluripotent archeocytes.
dc.format.extent13
dc.language.isoeng
dc.relation.ispartofEvoDevoen
dc.rightsCopyright the Author(s) 2016. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.en
dc.subjectPoriferaen
dc.subjectChoanocyteen
dc.subjectChoanocyte chamberen
dc.subjectDevelopmenten
dc.subjectDemospongiaeen
dc.subjectArcheocyteen
dc.subjectInvertebratesen
dc.subjectEvolutionen
dc.subjectStem cellsen
dc.subjectOntogenyen
dc.subjectGC Oceanographyen
dc.subjectQH301 Biologyen
dc.subject.lccGCen
dc.subject.lccQH301en
dc.titleThe ontogeny of choanocyte chambers during metamorphosis in the demosponge Amphimedon queenslandicaen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.Scottish Oceans Instituteen
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.identifier.doihttps://doi.org/10.1186/s13227-016-0042-x
dc.description.statusPeer revieweden


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