Show simple item record

Files in this item

Thumbnail

Item metadata

dc.contributor.authorMcCluskey, Kaley Allyn
dc.contributor.authorBoudreault, Julien
dc.contributor.authorSt-Pierre, Patrick
dc.contributor.authorPerez Gonzalez, Cibran
dc.contributor.authorChauvier, Adrien
dc.contributor.authorRizzi, Adrien
dc.contributor.authorBeauregard, Pascale B
dc.contributor.authorLafontaine, Daniel A
dc.contributor.authorPenedo-Esteiro, Juan Carlos
dc.date.accessioned2019-05-03T10:30:01Z
dc.date.available2019-05-03T10:30:01Z
dc.date.issued2019-07-09
dc.identifier.citationMcCluskey , K A , Boudreault , J , St-Pierre , P , Perez Gonzalez , C , Chauvier , A , Rizzi , A , Beauregard , P B , Lafontaine , D A & Penedo-Esteiro , J C 2019 , ' Unprecedented tunability of riboswitch structure and regulatory function by sub-millimolar variations in physiological Mg 2+ ' , Nucleic Acids Research , vol. 47 , no. 12 , pp. 6478–6487 . https://doi.org/10.1093/nar/gkz316en
dc.identifier.issn0305-1048
dc.identifier.otherPURE: 258630324
dc.identifier.otherPURE UUID: 25532c44-608f-4c62-ae82-2777522e9231
dc.identifier.otherScopus: 85069294521
dc.identifier.otherWOS: 000475891900041
dc.identifier.otherORCID: /0000-0002-5807-5385/work/74872772
dc.identifier.urihttps://hdl.handle.net/10023/17639
dc.description.abstractRiboswitches are cis-acting regulatory RNA biosensors that rival the efficiency of those found in proteins. At the heart of their regulatory function is the formation of a highly specific aptamer–ligand complex. Understanding how these RNAs recognize the ligand to regulate gene expression at physiological concentrations of Mg2+ ions and ligand is critical given their broad impact on bacterial gene expression and their potential as antibiotic targets. In this work, we used single-molecule FRET and biochemical techniques to demonstrate that Mg2+ ions act as fine-tuning elements of the amino acid-sensing lysC aptamer's ligand-free structure in the mesophile Bacillus subtilis. Mg2+ interactions with the aptamer produce encounter complexes with strikingly different sensitivities to the ligand in different, yet equally accessible, physiological ionic conditions. Our results demonstrate that the aptamer adapts its structure and folding landscape on a Mg2+-tunable scale to efficiently respond to changes in intracellular lysine of more than two orders of magnitude. The remarkable tunability of the lysC aptamer by sub-millimolar variations in the physiological concentration of Mg2+ ions suggests that some single-aptamer riboswitches have exploited the coupling of cellular levels of ligand and divalent metal ions to tightly control gene expression.
dc.format.extent10
dc.language.isoeng
dc.relation.ispartofNucleic Acids Researchen
dc.rights© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.subjectQH301 Biologyen
dc.subjectNDASen
dc.subject.lccQH301en
dc.titleUnprecedented tunability of riboswitch structure and regulatory function by sub-millimolar variations in physiological Mg2+en
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Physics and Astronomyen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews. Centre for Biophotonicsen
dc.identifier.doihttps://doi.org/10.1093/nar/gkz316
dc.description.statusPeer revieweden


This item appears in the following Collection(s)

Show simple item record