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dc.contributor.authorLongmuir, Sophie
dc.contributor.authorAkhtar, Nabihah
dc.contributor.authorMacNeill, Stuart Andrew
dc.date.accessioned2019-04-01T08:30:06Z
dc.date.available2019-04-01T08:30:06Z
dc.date.issued2019-03-29
dc.identifier258397551
dc.identifier3702981d-8242-4f11-bac2-45dd1a9c9c65
dc.identifier85063741565
dc.identifier000462719100005
dc.identifier.citationLongmuir , S , Akhtar , N & MacNeill , S A 2019 , ' Unexpected insertion of carrier DNA sequences into the fission yeast genome during CRISPR–Cas9 mediated gene deletion ' , BMC Research Notes , vol. 12 , 191 . https://doi.org/10.1186/s13104-019-4228-xen
dc.identifier.issn1756-0500
dc.identifier.otherORCID: /0000-0002-0555-0007/work/55901209
dc.identifier.urihttps://hdl.handle.net/10023/17406
dc.descriptionFunding: This work was funded by the School of Biology, University of St Andrews, and by the Nuffield Foundation.en
dc.description.abstractObjectives : The fission yeast Schizosaccharomyces pombe is predicted to encode ~ 200 proteins of < 100 amino acids, including a number of previously uncharacterised proteins that are found conserved in related Schizosaccharomyces species only. To begin an investigation of the function of four of these so-called microproteins (designated Smp1–Smp4), CRISPR–Cas9 genome editing technology was used to delete the corresponding genes in haploid fission yeast cells. Results : None of the four microprotein-encoding genes was essential for viability, meiosis or sporulation, and the deletion cells were no more sensitive to a range of cell stressors than wild-type, leaving the function of the proteins unresolved. During CRISPR–Cas9 editing however, a number of strains were isolated in which additional sequences were inserted into the target loci at the Cas9 cut site. Sequencing of the inserts revealed these to be derived from the chum salmon Oncorhynchus keta, the source of the carrier DNA used in the S. pombe transformation.
dc.format.extent5
dc.format.extent1087303
dc.language.isoeng
dc.relation.ispartofBMC Research Notesen
dc.subjectMicroproteinen
dc.subjectFission yeasten
dc.subjectSchizosaccharomyces pombeen
dc.subjectOncorhynchus ketaen
dc.subjectCRISPR–Cas9en
dc.subjectQH301 Biologyen
dc.subjectQH426 Geneticsen
dc.subjectNDASen
dc.subject.lccQH301en
dc.subject.lccQH426en
dc.titleUnexpected insertion of carrier DNA sequences into the fission yeast genome during CRISPR–Cas9 mediated gene deletionen
dc.typeJournal articleen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1186/s13104-019-4228-x
dc.description.statusPeer revieweden


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