Files in this item
The hydrophobic region of the Leishmania peroxin 14 : requirements for association with a glycosome mimetic membrane
Item metadata
dc.contributor.author | Cyr, Normand | |
dc.contributor.author | Smith, Terry K. | |
dc.contributor.author | Boisselier, Élodie | |
dc.contributor.author | Leroux, Louis-Philippe | |
dc.contributor.author | Kottarampatel, Anwar Hasil | |
dc.contributor.author | Davidsen, Amanda | |
dc.contributor.author | Salesse, Christian | |
dc.contributor.author | Jardim, Armando | |
dc.date.accessioned | 2019-01-31T00:33:44Z | |
dc.date.available | 2019-01-31T00:33:44Z | |
dc.date.issued | 2018-01-31 | |
dc.identifier | 253026328 | |
dc.identifier | 0f6713cd-105c-4938-8c0d-b27a9d13efe4 | |
dc.identifier | 000428085500009 | |
dc.identifier | 29259081 | |
dc.identifier | 85046670084 | |
dc.identifier | 000428085500009 | |
dc.identifier.citation | Cyr , N , Smith , T K , Boisselier , É , Leroux , L-P , Kottarampatel , A H , Davidsen , A , Salesse , C & Jardim , A 2018 , ' The hydrophobic region of the Leishmania peroxin 14 : requirements for association with a glycosome mimetic membrane ' , Biochemical Journal , vol. 475 , no. 2 , pp. 511-529 . https://doi.org/10.1042/BCJ20170746 | en |
dc.identifier.issn | 0264-6021 | |
dc.identifier.uri | https://hdl.handle.net/10023/16971 | |
dc.description | This work was funded by operating grants from the Canadian Institutes of Health Research (CIHR) and a Natural Sciences Engineering Research Council of Canada (NSERC) Discovery grant [Fonds de recherche du Québec — Nature et technologies (FRQNT) Regroupement Stratégique grant to the Centre for Host-Parasite Interactions (A.J.)]. N.C. was supported by a doctoral research scholarship from FRQNT. E.B. was supported by a Banting postdoctoral fellowship from CIHR. This work was also supported in part by Wellcome Trust grants [086658 and 093228] to T.K.S. C.S. recognizes the financial support from the Natural Sciences and Engineering Research Council of Canada and a Canada Foundation for Innovation grant [16299]. | en |
dc.description.abstract | Protein import into the Leishmania glycosome requires docking of the cargo-loaded peroxin 5 (PEX5) receptor to the peroxin 14 (PEX14) bound to the glycosome surface. To examine the LdPEX14-membrane interaction, we purified L. donovani promastigote glycosomes and determined the phospholipid and fatty acid composition. These membranes contained predominately phosphatidylethanolamine, phosphatidylcholine, and phosphatidylglycerol (PG) modified primarily with C18 and C22 unsaturated fatty acid. Using large unilamellar vesicles (LUVs) with a lipid composition mimicking the glycosomal membrane in combination with sucrose density centrifugation and fluorescence-activated cell sorting technique, we established that the LdPEX14 membrane-binding activity was dependent on a predicted transmembrane helix found within residues 149-179. Monolayer experiments showed that the incorporation of PG and phospholipids with unsaturated fatty acids, which increase membrane fluidity and favor a liquid expanded phase, facilitated the penetration of LdPEX14 into biological membranes. Moreover, we demonstrated that the binding of LdPEX5 receptor or LdPEX5-PTS1 receptor-cargo complex was contingent on the presence of LdPEX14 at the surface of LUVs. | |
dc.format.extent | 19 | |
dc.format.extent | 2152782 | |
dc.language.iso | eng | |
dc.relation.ispartof | Biochemical Journal | en |
dc.subject | Leishmania | en |
dc.subject | PEX14 | en |
dc.subject | Glycosome | en |
dc.subject | Membrane proteins | en |
dc.subject | Peroxin | en |
dc.subject | Transmembrane proteins | en |
dc.subject | QH426 Genetics | en |
dc.subject | QR Microbiology | en |
dc.subject | NDAS | en |
dc.subject.lcc | QH426 | en |
dc.subject.lcc | QR | en |
dc.title | The hydrophobic region of the Leishmania peroxin 14 : requirements for association with a glycosome mimetic membrane | en |
dc.type | Journal article | en |
dc.contributor.sponsor | The Wellcome Trust | en |
dc.contributor.sponsor | The Wellcome Trust | en |
dc.contributor.institution | University of St Andrews. School of Biology | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.identifier.doi | 10.1042/BCJ20170746 | |
dc.description.status | Peer reviewed | en |
dc.date.embargoedUntil | 2019-01-31 | |
dc.identifier.grantnumber | 086658 Z 08 Z | en |
dc.identifier.grantnumber | 093228/Z/10/Z | en |
This item appears in the following Collection(s)
Items in the St Andrews Research Repository are protected by copyright, with all rights reserved, unless otherwise indicated.