Show simple item record

Files in this item

Thumbnail

Item metadata

dc.contributor.authorMorton, Andrew
dc.contributor.authorMurawski, Caroline
dc.contributor.authorDeng, Yali
dc.contributor.authorKeum, Changmin
dc.contributor.authorMiles, Gareth B.
dc.contributor.authorTello, Javier A.
dc.contributor.authorGather, Malte C.
dc.date.accessioned2019-01-17T10:30:05Z
dc.date.available2019-01-17T10:30:05Z
dc.date.issued2019-03-18
dc.identifier.citationMorton , A , Murawski , C , Deng , Y , Keum , C , Miles , G B , Tello , J A & Gather , M C 2019 , ' Photostimulation for in vitro optogenetics with high power blue organic light-emitting diodes ' , Advanced Biosystems , vol. 3 , no. 3 , 1800290 . https://doi.org/10.1002/adbi.201800290en
dc.identifier.issn2366-7478
dc.identifier.otherPURE: 257258756
dc.identifier.otherPURE UUID: 6e6e145d-8d6a-4483-9d75-e06090b2f778
dc.identifier.otherORCID: /0000-0002-8624-4625/work/52888698
dc.identifier.otherORCID: /0000-0002-4857-5562/work/52888751
dc.identifier.otherScopus: 85063096130
dc.identifier.otherWOS: 000461872900009
dc.identifier.otherORCID: /0000-0001-6637-2155/work/64034497
dc.identifier.urihttp://hdl.handle.net/10023/16886
dc.descriptionFunding: Leverhulme Trust (RPG-2017-231), the EPSRC NSF-CBET lead agency agreement (EP/R010595/1, 1706207), the DARPA NESD programme (N66001-17-C-4012) and the RS Macdonald Charitable Trust. C.M. acknowledges funding by the European Commission through a Marie Sklodowska-Curie Individual Fellowship (703387). Y. L. Deng acknowledges support from the Chinese Scholarship Council (CSC).en
dc.description.abstractOptogenetics, photostimulation of neural tissues rendered sensitive to light, is widely used in neuroscience to modulate the electrical excitability of neurons. For effective optical excitation of neurons, light wavelength and power density must fit with the expression levels and biophysical properties of the genetically encoded light‐sensitive ion channels used to confer light sensitivity on cells—most commonly, channelrhodopsins (ChRs). As light sources, organic light‐emitting diodes (OLEDs) offer attractive properties for miniaturized implantable devices for in vivo optical stimulation, but they do not yet operate routinely at the optical powers required for optogenetics. Here, OLEDs with doped charge transport layers are demonstrated that deliver blue light with good stability over millions of pulses, at powers sufficient to activate the ChR, CheRiff when expressed in cultured primary neurons, allowing live cell imaging of neural activity with the red genetically encoded calcium indicator, jRCaMP1a. Intracellular calcium responses scale with the radiant flux of OLED emission, when varied through changes in the current density, number of pulses, frequency, and pulse width delivered to the devices. The reported optimization and characterization of high‐power OLEDs are foundational for the development of miniaturized OLEDs with thin‐layer encapsulation on bioimplantable devices to allow single‐cell activation in vivo.
dc.format.extent8
dc.language.isoeng
dc.relation.ispartofAdvanced Biosystemsen
dc.rightsCopyright © 2019 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.en
dc.subjectChannelrhodopsinen
dc.subjectOptogeneticsen
dc.subjectOrganic light-emitting diodesen
dc.subjectPhotostimulationen
dc.subjectQC Physicsen
dc.subjectQH301 Biologyen
dc.subjectRC0321 Neuroscience. Biological psychiatry. Neuropsychiatryen
dc.subjectT Technologyen
dc.subjectDASen
dc.subjectBDCen
dc.subject.lccQCen
dc.subject.lccQH301en
dc.subject.lccRC0321en
dc.subject.lccTen
dc.titlePhotostimulation for in vitro optogenetics with high power blue organic light-emitting diodesen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Physics and Astronomyen
dc.contributor.institutionUniversity of St Andrews.School of Psychology and Neuroscienceen
dc.contributor.institutionUniversity of St Andrews.Institute of Behavioural and Neural Sciencesen
dc.contributor.institutionUniversity of St Andrews.Cellular Medicine Divisionen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews.School of Medicineen
dc.contributor.institutionUniversity of St Andrews.Centre for Biophotonicsen
dc.identifier.doihttps://doi.org/10.1002/adbi.201800290
dc.description.statusPeer revieweden


This item appears in the following Collection(s)

Show simple item record