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Linear Eyring plots conceal a change in rate-limiting step in an enzyme reaction
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dc.contributor.author | Machado, Teresa Filipa Guerreiro | |
dc.contributor.author | Gloster, Tracey | |
dc.contributor.author | da Silva, Rafael Guimaraes | |
dc.date.accessioned | 2018-12-20T11:30:07Z | |
dc.date.available | 2018-12-20T11:30:07Z | |
dc.date.issued | 2018-12-11 | |
dc.identifier.citation | Machado , T F G , Gloster , T & da Silva , R G 2018 , ' Linear Eyring plots conceal a change in rate-limiting step in an enzyme reaction ' , Biochemistry , vol. 57 , no. 49 , pp. 6757-6761 . https://doi.org/10.1021/acs.biochem.8b01099 | en |
dc.identifier.issn | 0006-2960 | |
dc.identifier.other | PURE: 256704869 | |
dc.identifier.other | PURE UUID: fcc67f76-4d2f-46a1-b1b2-7a28a8904297 | |
dc.identifier.other | Scopus: 85058279472 | |
dc.identifier.other | ORCID: /0000-0002-1308-8190/work/51261120 | |
dc.identifier.other | WOS: 000453489500005 | |
dc.identifier.uri | https://hdl.handle.net/10023/16734 | |
dc.description | This work was supported by the Engineering and Physical Sciences Research Council (EPSRC) [grant number EP/L016419/1] via a CRITICAT Centre for Doctoral Training studentship to T.F.G.M. T.M.G. is a Wellcome Trust Career Development Fellow. | en |
dc.description.abstract | The temperature dependence of psychrophilic and mesophilic (R)-3-hydroxybutyrate dehydrogenase steady-state rates yields nonlinear and linear Eyring plots, respectively. Solvent viscosity effects and multiple- and single-turnover pre-steady-state kinetics demonstrate that while product release is rate-limiting at high temperatures for the psychrophilic enzyme, either interconversion between enzyme-substrates and enzyme-products complexes or a step prior to it limits the rate at low temperature. Unexpectedly, a similar change in rate-limiting step is observed with the mesophilic en-zyme, where a step prior to chemistry becomes rate-limiting at low temperature. This observation may have implica-tions for past and future interpretation of temperature-rate profiles. | |
dc.language.iso | eng | |
dc.relation.ispartof | Biochemistry | en |
dc.rights | © 2018 American Chemical Society. This is an open access article published under a Creative Commons Attribution (CC-BY) License, which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited. | en |
dc.subject | QD Chemistry | en |
dc.subject | QH301 Biology | en |
dc.subject | NDAS | en |
dc.subject.lcc | QD | en |
dc.subject.lcc | QH301 | en |
dc.title | Linear Eyring plots conceal a change in rate-limiting step in an enzyme reaction | en |
dc.type | Journal article | en |
dc.contributor.sponsor | EPSRC | en |
dc.contributor.sponsor | The Wellcome Trust | en |
dc.description.version | Publisher PDF | en |
dc.contributor.institution | University of St Andrews. School of Biology | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.identifier.doi | https://doi.org/10.1021/acs.biochem.8b01099 | |
dc.description.status | Peer reviewed | en |
dc.identifier.grantnumber | EP/L016419/1 | en |
dc.identifier.grantnumber | 095828/Z/11/Z | en |
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