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dc.contributor.authorGodin, Antoine G
dc.contributor.authorVarela, Juan A
dc.contributor.authorGao, Zhenghong
dc.contributor.authorDanné, Noémie
dc.contributor.authorDupuis, Julien P
dc.contributor.authorLounis, Brahim
dc.contributor.authorGroc, Laurent
dc.contributor.authorCognet, Laurent
dc.date.accessioned2018-12-17T10:30:04Z
dc.date.available2018-12-17T10:30:04Z
dc.date.issued2017-03
dc.identifier.citationGodin , A G , Varela , J A , Gao , Z , Danné , N , Dupuis , J P , Lounis , B , Groc , L & Cognet , L 2017 , ' Single-nanotube tracking reveals the nanoscale organization of the extracellular space in the live brain ' , Nature Nanotechnology , vol. 12 , no. 3 , pp. 238-243 . https://doi.org/10.1038/nnano.2016.248en
dc.identifier.issn1748-3387
dc.identifier.otherPURE: 256889478
dc.identifier.otherPURE UUID: 741f51b3-e41f-44a2-9af4-90a8f141a026
dc.identifier.otherPubMed: 27870840
dc.identifier.otherScopus: 85008702260
dc.identifier.otherORCID: /0000-0003-1901-1378/work/51700179
dc.identifier.urihttps://hdl.handle.net/10023/16704
dc.descriptionThis work was supported by CNRS, the Agence Nationale de la Recherche (ANR-14-OHRI-0001-01), IdEx Bordeaux (ANR-10-IDEX-03-02), Labex Brain (ANR-10-LABX-43), Conseil Régional d'Aquitaine (2011-1603009) and the France-BioImaging national infrastructure (ANR-10-INBS-04-01). A.G.G. acknowledges financial support from the Fondation pour la Recherche Médicale and the Fonds Recherche du Québec–Nature et Technologies. J.A.V. acknowledges funding from Marie Curie Individual Fellowship 326442.en
dc.description.abstractThe brain is a dynamic structure with the extracellular space (ECS) taking up almost a quarter of its volume. Signalling molecules, neurotransmitters and nutrients transit via the ECS, which constitutes a key microenvironment for cellular communication and the clearance of toxic metabolites. The spatial organization of the ECS varies during sleep, development and aging and is probably altered in neuropsychiatric and degenerative diseases, as inferred from electron microscopy and macroscopic biophysical investigations. Here we show an approach to directly observe the local ECS structures and rheology in brain tissue using super-resolution imaging. We inject single-walled carbon nanotubes into rat cerebroventricles and follow the near-infrared emission of individual nanotubes as they diffuse inside the ECS for tens of minutes in acute slices. Because of the interplay between the nanotube geometry and the ECS local environment, we can extract information about the dimensions and local viscosity of the ECS. We find a striking diversity of ECS dimensions down to 40 nm, and as well as of local viscosity values. Moreover, by chemically altering the extracellular matrix of the brains of live animals before nanotube injection, we reveal that the rheological properties of the ECS are affected, but these alterations are local and inhomogeneous at the nanoscale.
dc.format.extent6
dc.language.isoeng
dc.relation.ispartofNature Nanotechnologyen
dc.rightsCopyright © 2017 Macmillan Publishers Limited, part of Springer Nature. All rights reserved. This work has been made available online in accordance with the publisher’s policies. This is the author created, accepted version manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at https://doi.org/10.1038/nnano.2016.248en
dc.subjectQH301 Biologyen
dc.subjectNDASen
dc.subjectBDCen
dc.subjectR2Cen
dc.subject.lccQH301en
dc.titleSingle-nanotube tracking reveals the nanoscale organization of the extracellular space in the live brainen
dc.typeJournal articleen
dc.description.versionPostprinten
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.identifier.doihttps://doi.org/10.1038/nnano.2016.248
dc.description.statusPeer revieweden
dc.date.embargoedUntil2017-05-21


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