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dc.contributor.authorVarela, Juan A
dc.contributor.authorFerreira, Joana S
dc.contributor.authorDupuis, Julien P
dc.contributor.authorDurand, Pauline
dc.contributor.authorBouchet, Delphine
dc.contributor.authorGroc, Laurent
dc.date.accessioned2018-12-12T10:30:05Z
dc.date.available2018-12-12T10:30:05Z
dc.date.issued2016-10
dc.identifier.citationVarela , J A , Ferreira , J S , Dupuis , J P , Durand , P , Bouchet , D & Groc , L 2016 , ' Single nanoparticle tracking of N -methyl-d-aspartate receptors in cultured and intact brain tissue ' , Neurophotonics , vol. 3 , no. 4 , 041808 . https://doi.org/10.1117/1.NPh.3.4.041808en
dc.identifier.issn2329-423X
dc.identifier.otherPURE: 256889560
dc.identifier.otherPURE UUID: 549b59d1-449d-40ec-a305-130eb3ec3511
dc.identifier.otherPubMed: 27429996
dc.identifier.otherPubMedCentral: PMC4940612
dc.identifier.otherORCID: /0000-0003-1901-1378/work/51700175
dc.identifier.otherScopus: 84991525161
dc.identifier.urihttp://hdl.handle.net/10023/16671
dc.descriptionThis work was supported by the Centre National de la Recherche Scientifique (CNRS), Agence Nationale de la Recherche, Conseil Régional d’Aquitaine, and Marie Curie Individual Fellowship No. 326442.en
dc.description.abstractRecent developments in single-molecule imaging have revealed many biological mechanisms, providing high spatial and temporal resolution maps of molecular events. In neurobiology, these techniques unveiled that plasma membrane neurotransmitter receptors and transporters laterally diffuse at the surface of cultured brain cells. The photostability of bright nanoprobes, such as quantum dots (QDs), has given access to neurotransmitter receptor tracking over long periods of time with a high spatial resolution. However, our knowledge has been restricted to cultured systems, i.e., neurons and organotypic slices, therefore lacking several aspects of the intact brain rheology and connectivity. Here, we used QDs to track single glutamatergic N-methyl-d-aspartate receptors (NMDAR) in acute brain slices. By delivering functionalized nanoparticles in vivo through intraventricular injections to rats expressing genetically engineered-tagged NMDAR, we successfully tracked the receptors in native brain tissue. Comparing NMDAR tracking to different classical brain preparations (acute brain slices, cultured organotypic brain slices, and cultured neurons) revealed that the surface diffusion properties shared several features and are also influenced by the nature of the extracellular environment. Together, we describe the experimental procedures to track plasma membrane NMDAR in dissociated and native brain tissue, paving the way for investigations aiming at characterizing receptor diffusion biophysics in intact tissue and exploring the physiopathological roles of receptor surface dynamics.
dc.format.extent9
dc.language.isoeng
dc.relation.ispartofNeurophotonicsen
dc.rightsCopyright © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.en
dc.subjectRC0321 Neuroscience. Biological psychiatry. Neuropsychiatryen
dc.subjectNDASen
dc.subject.lccRC0321en
dc.titleSingle nanoparticle tracking of N-methyl-d-aspartate receptors in cultured and intact brain tissueen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.identifier.doihttps://doi.org/10.1117/1.NPh.3.4.041808
dc.description.statusPeer revieweden


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