Duplication of a single myhz1.1 gene facilitated the ability of goldfish (Carassius auratus) to alter fast muscle contractile properties with seasonal temperature change
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Seasonal temperature changes markedly effect the swimming performance of some cyprinid fish acutely tested at different temperatures, involving a restructuring of skeletalmuscle phenotype including changes in contractile properties and myosin heavy and light chain expression. We analyzed the transcriptome of fast myotomal muscle from goldfish (Carassius auratus L.) acclimated to either 8 or 25◦C for 4 weeks (12 h light:12 h dark) and identified 10 myosin heavy chains (myh) and 13 myosin light chain (myl) transcripts. Goldfish orthologs were classified based on zebrafish nomenclature as myhz1.1α, myhz1.1β, myhz1.1γ, myha, myhb, embryo_myh1, myh9b, smyh2, symh3, andmyh11 (myosin heavy chains) and myl1a, myl1b, myl2, myl9a, myl9b, myl3, myl13, myl6, myl12.1a, myl12.1b, myl12.2a, myl12.2b, and myl10 (myosin light chains). Themost abundantly expressed transcripts myhz1.1α, myhz1.1β, myhz1.1γ, myha, myl1a, myl1b, myl2, and myl3) were further investigated in fast skeletal muscle of goldfish acclimated to either 4, 8, 15, or 30◦C for 12 weeks (12 h light:12 h dark). Total copy number for the myosin heavy chains showed a distinct optimum at 15◦C (P<0.01). Together myhz1.1α and myhz1.1β comprised 90 to 97% of myhc transcripts below1 5◦C, but only 62% at 30◦C. Whereas myhz1.1α and myhz1.1β were equally abundant at 4 and 8◦C, myhz1.1β transcripts were 17 and 12 times higher than myhz1.1α at 15 and 30◦C, respectively, (P<0.01). Myhz1.1γ expression was at least nine-fold higher at 30◦C than at cooler temperatures (P<0.01). In contrast, the expression of myha and myosin light chains showed no consistent pattern with acclimation temperature. A phylogenetic analysis indicated that the previously reported ability of goldfish and common carp to alter contractile properties and myofibrillar ATPase activity with temperature acclimation was related to the duplication of a single myhz1.1 fast muscle myosin heavy chain found in basal cyprinids such as the zebrafish (Danio rerio).
Garcia de la Serrana , D , Wreggelsworth , K & Johnston , I A 2018 , ' Duplication of a single myhz1.1 gene facilitated the ability of goldfish ( Carassius auratus ) to alter fast muscle contractile properties with seasonal temperature change ' , Frontiers in Physiology , vol. 9 , 1724 . https://doi.org/10.3389/fphys.2018.01724
Frontiers in Physiology
Copyright © 2018 Garcia de la serrana, Wreggelsworth and Johnston. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
DescriptionThis work received funding from the MASTS pooling initiative (The Marine Alliance for Science and Technology for Scotland) and their support was gratefully acknowledged. MASTS was funded by the Scottish Funding Council (Grant Reference HR09011) and contributing institutions.
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