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dc.contributor.authorAntoniou, Antony N.
dc.contributor.authorLenart, Izabela
dc.contributor.authorKriston-Vizi, Janos
dc.contributor.authorIwawaki, Takao
dc.contributor.authorTurmaine, Mark
dc.contributor.authorMcHugh, Kirsty
dc.contributor.authorAli, Sadfer
dc.contributor.authorBlake, Neil
dc.contributor.authorBowness, Paul
dc.contributor.authorBajaj-Elliott, Mona
dc.contributor.authorGould, Keith
dc.contributor.authorNesbeth, Darren
dc.contributor.authorPowis, Simon John
dc.identifier.citationAntoniou , A N , Lenart , I , Kriston-Vizi , J , Iwawaki , T , Turmaine , M , McHugh , K , Ali , S , Blake , N , Bowness , P , Bajaj-Elliott , M , Gould , K , Nesbeth , D & Powis , S J 2019 , ' Salmonella exploits HLA-B27 and host unfolded protein responses to promote intracellular replication ' , Annals of the Rheumatic Diseases , vol. 78 , no. 1 , 213532 , pp. 74–82 .
dc.identifier.otherPURE: 255169754
dc.identifier.otherPURE UUID: f4ff63c7-eb89-4a36-8344-98e8062b8109
dc.identifier.otherScopus: 85055489648
dc.identifier.otherORCID: /0000-0003-4218-2984/work/60195301
dc.identifier.otherWOS: 000455953500022
dc.descriptionA.N.A was funded by ARUK Fellowships Non-Clinical Career Development Fellowship Ref No: 18440. I.L. was funded by an ARUK PhD studentship Ref No: 17868. A.N.A and S.J.P were also in part funded by ARUK (grant 21261)en
dc.description.abstractObjective Salmonella enterica infections can lead to Reactive Arthritis (ReA), which can exhibit an association with human leucocyte antigen (HLA)-B*27:05, a molecule prone to misfolding and initiation of the unfolded protein response (UPR). This study examined how HLA-B*27:05 expression and the UPR affect the Salmonella life-cycle within epithelial cells. Methods Isogenic epithelial cell lines expressing two copies of either HLA-B*27:05 and a control HLA-B*35:01 heavy chain (HC) were generated to determine the effect on the Salmonella infection life-cycle. A cell line expressing HLA-B*27:05.HC physically linked to the light chain beta-2-microglobulin and a specific peptide (referred to as a single chain trimer, SCT) was also generated to determine the effects of HLA-B27 folding status on S. enterica life-cycle. XBP-1 venus and AMP dependent Transcription Factor (ATF6)-FLAG reporters were used to monitor UPR activation in infected cells. Triacin C was used to inhibit de novo lipid synthesis during UPR, and confocal imaging of ER tracker stained membrane allowed quantification of glibenclamide-associated membrane. Results S. enterica demonstrated enhanced replication with an altered cellular localisation in the presence of HLA-B*27:05.HC but not in the presence of HLA-B*27:05.SCT or HLA-B*35:01. HLA-B*27:05.HC altered the threshold for UPR induction. Salmonella activated the UPR and required XBP-1 for replication, which was associated with endoreticular membrane expansion and lipid metabolism. Conclusions HLA-B27 misfolding and a UPR cellular environment are associated with enhanced Salmonella replication, while Salmonella itself can activate XBP-1 and ATF6. These data provide a potential mechanism linking the life-cycle of Salmonella with the physicochemical properties of HLA-B27 and cellular events that may contribute to ReA pathogenesis. Our observations suggest that the UPR pathway maybe targeted for future therapeutic intervention.
dc.relation.ispartofAnnals of the Rheumatic Diseasesen
dc.rights© Author(s) (or their employer(s)) 2018. This is an open access article distributed in accordance with the Creative Commons Attribution 4.0 Unported (CC BY 4.0) license, which permits others to copy, redistribute, remix, transform and build upon this work for any purpose, provided the original work is properly cited, a link to the licence is given, and indication of whether changes were made. See:
dc.subjectQR Microbiologyen
dc.subjectRC Internal medicineen
dc.titleSalmonella exploits HLA-B27 and host unfolded protein responses to promote intracellular replicationen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.Cellular Medicine Divisionen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews.School of Medicineen
dc.contributor.institutionUniversity of St Andrews.Centre for Biophotonicsen
dc.description.statusPeer revieweden

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