Show simple item record

Files in this item

Thumbnail

Item metadata

dc.contributor.authorWang, Shumei
dc.contributor.authorWelsh, Lydia
dc.contributor.authorThorpe, Peter
dc.contributor.authorWhisson, Stephen C
dc.contributor.authorBoevink, Petra C
dc.contributor.authorBirch, Paul R J
dc.date.accessioned2018-10-05T14:30:09Z
dc.date.available2018-10-05T14:30:09Z
dc.date.issued2018-08-28
dc.identifier.citationWang , S , Welsh , L , Thorpe , P , Whisson , S C , Boevink , P C & Birch , P R J 2018 , ' The Phytophthora infestans haustorium is a site for secretion of diverse classes of infection-associated proteins ' , mBio , vol. 9 , no. 4 , e01216-18 . https://doi.org/10.1128/mBio.01216-18en
dc.identifier.issn2150-7511
dc.identifier.otherPURE: 256115934
dc.identifier.otherPURE UUID: c0d4926d-1fb0-4384-8321-2bc61088157a
dc.identifier.otherPubMed: 30154258
dc.identifier.otherPubMedCentral: PMC6113627
dc.identifier.otherScopus: 85055556937
dc.identifier.urihttp://hdl.handle.net/10023/16153
dc.descriptionWe are grateful to the China Scholarship Council for funds to support S.W. and to the Biotechnology and Biological Sciences Research Council (BBSRC) (grants BB/N009967/1, BB/L026880/1, and BB/J016500/1) and the Scottish Government Rural and Environment Science and Analytical Services Division (RESAS) for funding provided to P.R.J.B., P.C.B., L.W., and S.C.W.en
dc.description.abstractThe oomycete potato blight pathogen Phytophthora infestans secretes a diverse set of proteins to manipulate host plant immunity. However, there is limited knowledge about how and where they are secreted during infection. Here we used the endoplasmic reticulum (ER)-to-Golgi secretion pathway inhibitor brefeldin A (BFA) in combination with liquid chromatography-electrospray tandem mass spectrometry (LC-MS/MS) to identify extracellular proteins from P. infestans that were conventionally secreted from in vitro-cultured hyphae. We identified 19 proteins with predicted signal peptides that potentially influence plant interactions for which secretion was attenuated by BFA. In addition to inhibition by the apoplastic effector EPIC1, a cysteine protease inhibitor, we show that secretion of the cell wall-degrading pectinesterase enzyme PE1 and the microbe-associated molecular pattern (MAMP)-like elicitin INF4 was inhibited by BFA in vitro and in planta, demonstrating that these proteins are secreted by the conventional, Golgi-mediated pathway. For comparison, secretion of a cytoplasmic RXLR (Arg-[any amino acid]-Leu-Arg) effector, Pi22926, was not inhibited by BFA. During infection, whereas INF4 accumulated outside the plant cell, RXLR effector Pi22926 entered the plant cell and accumulated in the nucleus. The P. infestans effectors, the PE1 enzyme, and INF4 were all secreted from haustoria, pathogen structures that penetrate the plant cell wall to form an intimate interaction with the host plasma membrane. Our findings show the haustorium to be a major site of both conventional and nonconventional secretion of proteins with diverse functions during infection.
dc.format.extent14
dc.language.isoeng
dc.relation.ispartofmBioen
dc.rightsCopyright © 2018 Wang (王姝梅) et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.en
dc.subjectCrop diseaseen
dc.subjectFilamentous plant pathogenen
dc.subjectPathogenicityen
dc.subjectSecretomeen
dc.subjectVirulenceen
dc.subjectQH301 Biologyen
dc.subjectQR Microbiologyen
dc.subjectDASen
dc.subject.lccQH301en
dc.subject.lccQRen
dc.titleThe Phytophthora infestans haustorium is a site for secretion of diverse classes of infection-associated proteinsen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Medicineen
dc.identifier.doihttps://doi.org/10.1128/mBio.01216-18
dc.description.statusPeer revieweden


This item appears in the following Collection(s)

Show simple item record