Show simple item record

Files in this item

Thumbnail

Item metadata

dc.contributor.authorHarkins, C. P.
dc.contributor.authorMcAleer, M. A.
dc.contributor.authorBennett, D.
dc.contributor.authorMcHugh, M.
dc.contributor.authorFleury, O. M.
dc.contributor.authorPettigrew, K. A.
dc.contributor.authorOravcová, K.
dc.contributor.authorParkhill, J.
dc.contributor.authorProby, C. M.
dc.contributor.authorDawe, R. S.
dc.contributor.authorGeoghegan, J. A.
dc.contributor.authorIrvine, A. D.
dc.contributor.authorHolden, M. T. G.
dc.date.accessioned2018-07-27T15:30:05Z
dc.date.available2018-07-27T15:30:05Z
dc.date.issued2018-07-24
dc.identifier.citationHarkins , C P , McAleer , M A , Bennett , D , McHugh , M , Fleury , O M , Pettigrew , K A , Oravcová , K , Parkhill , J , Proby , C M , Dawe , R S , Geoghegan , J A , Irvine , A D & Holden , M T G 2018 , ' The widespread use of topical antimicrobials enriches for resistance in  Staphylococcus aureus isolated from Atopic Dermatitis patients ' , British Journal of Dermatology , vol. Early View . https://doi.org/10.1111/bjd.16722en
dc.identifier.issn0007-0963
dc.identifier.otherPURE: 253026549
dc.identifier.otherPURE UUID: a8dfb9a2-0f2f-4c41-9658-bda7556c3100
dc.identifier.otherBibtex: urn:4433c2051b97a43c1ed8632f1525ce12
dc.identifier.otherScopus: 85054749472
dc.identifier.otherORCID: /0000-0002-0370-3700/work/46939700
dc.identifier.otherORCID: /0000-0002-4958-2166/work/60196364
dc.identifier.otherWOS: 000448855500034
dc.identifier.urihttp://hdl.handle.net/10023/15721
dc.descriptionThis work was supported by grants from the Wellcome Trust (104241/z/14/z to C.P.H., and 098731/z/11/z to St Andrews University Bioinformatics Unit), and the Chief Scientists Office (SIRN10 to M.T.G.H.).en
dc.description.abstractBackground: Carriage rates of Staphylococcus aureus on affected skin in atopic dermatitis (AD) are approximately 70 Increasing disease severity during flares and overall disease severity correlate with increased burden of S. aureus. Treatment in AD therefore often targets S. aureus, with topical and systemic antimicrobials. Objectives: To determine if antimicrobial sensitivities and genetic determinants of resistance differed in S. aureus isolates from the skin of children with AD compared with healthy child nasal carriers. Methods:  In this case‐control study, we compared S. aureus isolates from children with AD (n=50) attending a hospital dermatology department to nasal carriage isolates from children without skin disease (n=49) attending a hospital emergency department for non‐infective conditions. Using whole genome sequencing we generated a phylogenetic framework for the isolates based on variation in the core genome, then compared antimicrobial resistance phenotype and genotypes between disease groups. Results and conclusions:  S. aureus from cases and controls had on average similar numbers of phenotypic resistances per isolate. Case isolates differed in their resistance patterns, with Fusidic acid resistance (FusR) being significantly more frequent in AD (p=0.009). The genetic basis of FusR also differentiated the populations, with chromosomal mutations in fusA predominating in AD (p=0.049). Analysis revealed that FusR evolved multiple times and via multiple mechanism in the population. Carriage of plasmid derived qac genes, which have been associated with reduced susceptibility to antiseptics, was 8 times more frequent in AD (p=0.016). The results suggest strong selective pressure drives the emergence and maintenance of specific resistances in AD.
dc.format.extent8
dc.language.isoeng
dc.relation.ispartofBritish Journal of Dermatologyen
dc.rights© 2018 The Authors. British Journal of Dermatology published by John Wiley & Sons Ltd on behalf of British Association of Dermatologists. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.en
dc.subjectRJ101 Child Health. Child health servicesen
dc.subjectRL Dermatologyen
dc.subjectQH426 Geneticsen
dc.subjectDASen
dc.subject.lccRJ101en
dc.subject.lccRLen
dc.subject.lccQH426en
dc.titleThe widespread use of topical antimicrobials enriches for resistance in Staphylococcus aureus isolated from Atopic Dermatitis patientsen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Medicineen
dc.contributor.institutionUniversity of St Andrews.Infection and Global Health Divisionen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews.Infection Groupen
dc.identifier.doihttps://doi.org/10.1111/bjd.16722
dc.description.statusPeer revieweden


This item appears in the following Collection(s)

Show simple item record