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dc.contributor.authorAfshar Saber, Wardiya
dc.contributor.authorGasparoli, Federico M.
dc.contributor.authorDirks, Marjet Gabrielle
dc.contributor.authorGunn-Moore, Frank J.
dc.contributor.authorAntkowiak, Maciej
dc.date.accessioned2018-07-10T08:30:13Z
dc.date.available2018-07-10T08:30:13Z
dc.date.issued2018-07-05
dc.identifier.citationAfshar Saber , W , Gasparoli , F M , Dirks , M G , Gunn-Moore , F J & Antkowiak , M 2018 , ' All-optical assay to study biological neural networks ' Frontiers in Neuroscience , vol. 12 , 451 . https://doi.org/10.3389/fnins.2018.00451en
dc.identifier.issn1662-453X
dc.identifier.otherPURE: 253391231
dc.identifier.otherPURE UUID: 67dc8d29-c4e1-483d-8add-a693796f9ce0
dc.identifier.otherScopus: 85049995354
dc.identifier.otherORCID: /0000-0003-3422-3387/work/46569122
dc.identifier.otherWOS: 000437466100001
dc.identifier.urihttp://hdl.handle.net/10023/15153
dc.descriptionThis work was supported by the Cunningham Trust Ph.D. Studentship, the RS MacDonald Charitable Trust Neurophotonics Grant and EPSRC programme Grant EP/P030017/1.en
dc.description.abstractWe introduce a novel all-optical assay for functional studies of biological neural networks in vitro. We created a novel optogenetics construct named OptoCaMP which is a combination of a channelrhodopsin variant (CheRiff) and a red genetically encoded calcium indicator (jRCamp1b). It enables simultaneous optical stimulation and recording from large population of neurons with single-cell readout. We also developed a spatio-temporal all-optical assay to simultaneously stimulate a sub-section of a neural network and record evoked calcium activity, in both stimulated and non-stimulated neurons, thus allowing the investigation of the spread of excitation through an interconnected network. Finally, we demonstrate the sensitivity of this assay to the change of neural network connectivity.
dc.format.extent12
dc.language.isoeng
dc.relation.ispartofFrontiers in Neuroscienceen
dc.rights© 2018 Afshar Saber, Gasparoli, Dirks, Gunn-Moore and Antkowiak. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.subjectAll-optical assayen
dc.subjectOptogeneticsen
dc.subjectCalcium imagingen
dc.subjectNeural networksen
dc.subjectConnectivityen
dc.subjectNeuronsen
dc.subjectLentiviral transductionen
dc.subjectRC0321 Neuroscience. Biological psychiatry. Neuropsychiatryen
dc.subjectNDASen
dc.subject.lccRC0321en
dc.titleAll-optical assay to study biological neural networksen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Medicineen
dc.contributor.institutionUniversity of St Andrews.School of Physics and Astronomyen
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.contributor.institutionUniversity of St Andrews.Institute of Behavioural and Neural Sciencesen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.3389/fnins.2018.00451
dc.description.statusPeer revieweden


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