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All-optical assay to study biological neural networks
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dc.contributor.author | Afshar Saber, Wardiya | |
dc.contributor.author | Gasparoli, Federico M. | |
dc.contributor.author | Dirks, Marjet Gabrielle | |
dc.contributor.author | Gunn-Moore, Frank J. | |
dc.contributor.author | Antkowiak, Maciej | |
dc.date.accessioned | 2018-07-10T08:30:13Z | |
dc.date.available | 2018-07-10T08:30:13Z | |
dc.date.issued | 2018-07-05 | |
dc.identifier | 253391231 | |
dc.identifier | 67dc8d29-c4e1-483d-8add-a693796f9ce0 | |
dc.identifier | 85049995354 | |
dc.identifier | 000437466100001 | |
dc.identifier.citation | Afshar Saber , W , Gasparoli , F M , Dirks , M G , Gunn-Moore , F J & Antkowiak , M 2018 , ' All-optical assay to study biological neural networks ' , Frontiers in Neuroscience , vol. 12 , 451 . https://doi.org/10.3389/fnins.2018.00451 | en |
dc.identifier.issn | 1662-453X | |
dc.identifier.other | ORCID: /0000-0003-3422-3387/work/46569122 | |
dc.identifier.uri | https://hdl.handle.net/10023/15153 | |
dc.description | This work was supported by the Cunningham Trust Ph.D. Studentship, the RS MacDonald Charitable Trust Neurophotonics Grant and EPSRC programme Grant EP/P030017/1. | en |
dc.description.abstract | We introduce a novel all-optical assay for functional studies of biological neural networks in vitro. We created a novel optogenetics construct named OptoCaMP which is a combination of a channelrhodopsin variant (CheRiff) and a red genetically encoded calcium indicator (jRCamp1b). It enables simultaneous optical stimulation and recording from large population of neurons with single-cell readout. We also developed a spatio-temporal all-optical assay to simultaneously stimulate a sub-section of a neural network and record evoked calcium activity, in both stimulated and non-stimulated neurons, thus allowing the investigation of the spread of excitation through an interconnected network. Finally, we demonstrate the sensitivity of this assay to the change of neural network connectivity. | |
dc.format.extent | 12 | |
dc.format.extent | 2577805 | |
dc.language.iso | eng | |
dc.relation.ispartof | Frontiers in Neuroscience | en |
dc.subject | All-optical assay | en |
dc.subject | Optogenetics | en |
dc.subject | Calcium imaging | en |
dc.subject | Neural networks | en |
dc.subject | Connectivity | en |
dc.subject | Neurons | en |
dc.subject | Lentiviral transduction | en |
dc.subject | RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry | en |
dc.subject | NDAS | en |
dc.subject.lcc | RC0321 | en |
dc.title | All-optical assay to study biological neural networks | en |
dc.type | Journal article | en |
dc.contributor.sponsor | EPSRC | en |
dc.contributor.sponsor | EPSRC | en |
dc.contributor.institution | University of St Andrews. School of Medicine | en |
dc.contributor.institution | University of St Andrews. School of Physics and Astronomy | en |
dc.contributor.institution | University of St Andrews. School of Biology | en |
dc.contributor.institution | University of St Andrews. Institute of Behavioural and Neural Sciences | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.identifier.doi | 10.3389/fnins.2018.00451 | |
dc.description.status | Peer reviewed | en |
dc.identifier.grantnumber | EP/P030017/1 | en |
dc.identifier.grantnumber | EP/P030017/1 | en |
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