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dc.contributor.authorHulme, Charlotte H.
dc.contributor.authorWilson, Emma L.
dc.contributor.authorFuller, Heidi R.
dc.contributor.authorRoberts, Sally
dc.contributor.authorRichardson, James B.
dc.contributor.authorGallacher, Pete
dc.contributor.authorPeffers, Mandy J.
dc.contributor.authorShirran, Sally L.
dc.contributor.authorBotting, Catherine H.
dc.contributor.authorWright, Karina T.
dc.date.accessioned2018-05-03T11:30:11Z
dc.date.available2018-05-03T11:30:11Z
dc.date.issued2018-05-02
dc.identifier.citationHulme , C H , Wilson , E L , Fuller , H R , Roberts , S , Richardson , J B , Gallacher , P , Peffers , M J , Shirran , S L , Botting , C H & Wright , K T 2018 , ' Two independent proteomic approaches provide a comprehensive analysis of the synovial fluid proteome response to Autologous Chondrocyte Implantation ' , Arthritis Research & Therapy , vol. 20 , 87 . https://doi.org/10.1186/s13075-018-1573-4en
dc.identifier.issn1478-6362
dc.identifier.otherPURE: 252995801
dc.identifier.otherPURE UUID: 9a8a9986-d688-4848-9b27-27b98fc8d740
dc.identifier.otherRIS: urn:74D5C64CF4EA357F90780AEBBAAFF773
dc.identifier.otherRIS: Hulme2018
dc.identifier.otherScopus: 85046257550
dc.identifier.otherORCID: /0000-0003-3516-3507/work/44361957
dc.identifier.otherWOS: 000431295100011
dc.identifier.urihttp://hdl.handle.net/10023/13288
dc.descriptionWe thank Arthritis Research UK for supporting this work via grants 19429, 20815 and 21122. MJP is supported through a Wellcome Trust Clinical Intermediate Fellowship. This work was supported by Wellcome Trust grant 094476/Z/10/Z, which funded the purchase of the TripleTOF 5600 mass spectrometer at the Biomedical Sciences Research Complex Mass Spectrometry and Proteomics Facility, University of St. Andrews (Fife, UK).en
dc.description.abstractAutologous chondrocyte implantation (ACI) has a failure rate of approximately 20%, but it is yet to be fully understood why. Biomarkers are needed that can pre-operatively predict in which patients it is likely to fail, so that alternative or individualised therapies can be offered. We previously used label-free quantitation (LF) with a dynamic range compression proteomic approach to assess the synovial fluid (SF) of ACI responders and non-responders. However, we were able to identify only a few differentially abundant proteins at baseline. In the present study, we built upon these previous findings by assessing higher-abundance proteins within this SF, providing a more global proteomic analysis on the basis of which more of the biology underlying ACI success or failure can be understood.
dc.format.extent17
dc.language.isoeng
dc.relation.ispartofArthritis Research & Therapyen
dc.rights© The Author(s). 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.en
dc.subjectAutologous chondrocyte implantation (ACI)en
dc.subjectiTRAQ proteomicsen
dc.subjectLabel-free quantitation proteomicsen
dc.subjectSynovial fluiden
dc.subjectCartilage repairen
dc.subjectComplement C1S subcomponenten
dc.subjectMatrix metalloproteinase 3en
dc.subjectMMP3en
dc.subjectR Medicineen
dc.subjectQH301 Biologyen
dc.subjectDASen
dc.subject.lccRen
dc.subject.lccQH301en
dc.titleTwo independent proteomic approaches provide a comprehensive analysis of the synovial fluid proteome response to Autologous Chondrocyte Implantationen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews.School of Chemistryen
dc.contributor.institutionUniversity of St Andrews.EaSTCHEMen
dc.identifier.doihttps://doi.org/10.1186/s13075-018-1573-4
dc.description.statusPeer revieweden


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