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dc.contributor.authorO’Leary, Shannon J.
dc.contributor.authorHollenbeck, Christopher M.
dc.contributor.authorVega, Robert R.
dc.contributor.authorGold, John R.
dc.contributor.authorPortnoy, David S.
dc.date.accessioned2018-04-12T14:30:53Z
dc.date.available2018-04-12T14:30:53Z
dc.date.issued2018-02-23
dc.identifier.citationO’Leary , S J , Hollenbeck , C M , Vega , R R , Gold , J R & Portnoy , D S 2018 , ' Genetic mapping and comparative genomics to inform restoration enhancement and culture of southern flounder, Paralichthys lethostigma ' BMC Genomics , vol. 19 , 163 . https://doi.org/10.1186/s12864-018-4541-0en
dc.identifier.issn1471-2164
dc.identifier.otherPURE: 252804751
dc.identifier.otherPURE UUID: c554cbe7-20a5-4a6a-b252-e1da90520de7
dc.identifier.otherRIS: urn:4F760A937B7A6772C557A384BD423FA3
dc.identifier.otherScopus: 85042405357
dc.identifier.urihttp://hdl.handle.net/10023/13126
dc.descriptionFunds were provided by Texas Parks and Wildlife and the U.S. Fish and Wildlife Service through a Wildlife & Sport Fish Restoration State Wildlife Grant (TX T-112).en
dc.description.abstractBackground:  Southern flounder, Paralichthys lethostigma, historically support a substantial fishery along the Atlantic and Gulf coasts of the southern United States. Low year-class strengths over the past few years in the western Gulf of Mexico have raised concern that spawning stocks may be overfished. Current management of the resource includes releasing hatchery-raised juveniles to restock bays and estuaries; additionally, there is a growing interest in the potential for commercial aquaculture of the species. Currently, genomic resources for southern flounder do not exist. Here, we used two hatchery-reared families and double-digest, restriction-site-associated DNA (ddRAD) sequencing to create a reduced-representation genomic library consisting of several thousand single nucleotide polymorphisms (SNPs) located throughout the genome. Results: The relative position of each SNP-containing locus was determined to create a high-density genetic map spanning the 24 linkage groups of the southern flounder genome. The consensus map was used to identify regions of shared synteny between southern flounder and seven other fish species for which genome assemblies are available. Finally, syntenic blocks were used to localize genes identified from transcripts in European flounder as potentially being involved in ecotoxicological and osmoregulatory responses, as well as QTLs associated with growth and disease resistance in Japanese flounder, on the southern flounder linkage map. Conclusions:  The information provided by the linkage map will enrich restoration efforts by providing a foundation for interpreting spatial genetic variation within the species, ultimately furthering an understanding of the adaptive potential and resilience of southern flounder to future changes in local environmental conditions. Further, the map will facilitate the use of genetic markers to enhance restoration and commercial aquaculture.en
dc.format.extent11en
dc.language.isoeng
dc.relation.ispartofBMC Genomicsen
dc.rights© The Author(s). 2018. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.en
dc.subjectLinkage mapen
dc.subjectRADseqen
dc.subjectAquacultureen
dc.subjectSyntenyen
dc.subjectQH301 Biologyen
dc.subjectQH426 Geneticsen
dc.subjectSH Aquaculture. Fisheries. Anglingen
dc.subjectDASen
dc.subject.lccQH301en
dc.subject.lccQH426en
dc.subject.lccSHen
dc.titleGenetic mapping and comparative genomics to inform restoration enhancement and culture of southern flounder, Paralichthys lethostigmaen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.identifier.doihttps://doi.org/10.1186/s12864-018-4541-0
dc.description.statusPeer revieweden


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