Show simple item record

Files in this item

Thumbnail

Item metadata

dc.contributor.authorKumwenda, Geoffrey P.
dc.contributor.authorChipungu, Geoffrey
dc.contributor.authorSloan, Derek J.
dc.contributor.authorKaimila, Yankho
dc.contributor.authorChiumya, Kondwani
dc.contributor.authorPangani, Harry
dc.date.accessioned2018-04-10T14:30:07Z
dc.date.available2018-04-10T14:30:07Z
dc.date.issued2018-03
dc.identifier.citationKumwenda , G P , Chipungu , G , Sloan , D J , Kaimila , Y , Chiumya , K & Pangani , H 2018 , ' The occurrence and frequency of genomic mutations that mediate Isoniazid and Rifampicin resistance in Mycobacterium tuberculosis isolates from untreated pulmonary Tuberculosis cases in urban Blantyre, Malawi ' , Malawi Medical Journal , vol. 30 , no. 1 , pp. 1-5 . https://doi.org/10.4314/mmj.v30i1.1en
dc.identifier.issn1995-7262
dc.identifier.otherPURE: 252782793
dc.identifier.otherPURE UUID: fb5a5e69-ce1b-4b1c-b4e9-d9d256af7cc6
dc.identifier.otherWOS: 000428140000001
dc.identifier.otherScopus: 85044752404
dc.identifier.otherWOS: 000428140000001
dc.identifier.otherORCID: /0000-0002-7888-5449/work/60631018
dc.identifier.urihttp://hdl.handle.net/10023/13108
dc.descriptionThis study was funded by the Helse Nord Tuberculosis Initiative (HNTI), a College of Medicine grant supported by the Helse Nord RHF and the University of Tromso.en
dc.description.abstractBackground The emergence and spread of drug-resistant Tuberculosis (TB) is a major public health threat. TB resistance originates in the course of treatment due to genomic mutations in Mycobacterium tuberculosis (MTB). An increase in new cases with drug-resistant TB could be an indicator of high levels of circulating resistant strains. This study was conducted to determine the occurrence and frequency of genomic mutations that mediate Isoniazid (INH) and Rifampicin (RIF) resistance among isolates from untreated TB cases in urban Blantyre, Malawi. Methods A cross-sectional retrospective study was conducted on a panel of 141(n=141) MTB clinical isolates recovered between June 2010 and January 2012 from ≥2+ Ziehl-Neelsen smear positive new pulmonary-TB patients with no history of treatment. Frozen isolates were revived using the BACTEC MGIT detection system. DNA was extracted using GenoLyse DNA extraction kit and detection of genomic mutations was carried out using the GenoType MTBDRplus Ver 2.0 assay. Results Out of the 141 isolates studied, 3 (2.1%) were found carrying mutations in the katG gene that confer resistance to Isoniazid (INH). No mutations were detected in the inhA promoter region gene that confer weak INH resistance or in the rpoB gene that confer Rifampicin resistance. All katG mutant genes had a S315T1 single point mutation, a genomic alteration that mediates high INH resistance. Conclusion The katG mutant gene conferring resistance to INH was the only genomic mutation observed among the isolates studied and the frequency of occurrence was low. Our findings suggest low levels of circulating drug-resistant MTB strains in urban Blantyre, Malawi.
dc.format.extent5
dc.language.isoeng
dc.relation.ispartofMalawi Medical Journalen
dc.rights© 2018 The College of Medicine and the Medical Association of Malawi. This work is licensed under the Creative Commons Attribution 4.0 International License. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)en
dc.subjectQH426 Geneticsen
dc.subjectRA0421 Public health. Hygiene. Preventive Medicineen
dc.subjectNDASen
dc.subject.lccQH426en
dc.subject.lccRA0421en
dc.titleThe occurrence and frequency of genomic mutations that mediate Isoniazid and Rifampicin resistance in Mycobacterium tuberculosis isolates from untreated pulmonary Tuberculosis cases in urban Blantyre, Malawien
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Medicineen
dc.contributor.institutionUniversity of St Andrews. Infection and Global Health Divisionen
dc.identifier.doihttps://doi.org/10.4314/mmj.v30i1.1
dc.description.statusPeer revieweden
dc.identifier.urlhttp://www.mmj.mw/?p=6703en


This item appears in the following Collection(s)

Show simple item record