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Blocking variant surface glycoprotein synthesis alters endoplasmic reticulum exit sites/Golgi homeostasis in Trypanosoma brucei

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Ooi_2018_Traffic_Endoplasmicreticulum_CC.pdf (32.71Mb)
Date
06/04/2018
Author
Ooi, Cher-Pheng
Smith, Terry K.
Gluenz, Eva
Wand, Nadina Vasileva
Vaughan, Sue
Rudenko, Gloria
Keywords
Golgi biogenesis
Secretory pathway
Trypanosoma brucei
Variant Surface Glycoprotein
Membrane metabolism
ER exit site
QH301 Biology
NDAS
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Abstract
The predominant secretory cargo of bloodstream form Trypanosoma brucei is Variant Surface Glycoprotein (VSG), comprising ~10% total protein and forming a dense protective layer. Blocking VSG translation using Morpholino oligonucleotides triggered a precise pre-cytokinesis arrest. We investigated the effect of blocking VSG synthesis on the secretory pathway. The number of Golgi decreased, particularly in post-mitotic cells, from 3.5 ± 0.6 to 2.0 ± 0.04 per cell. Similarly, the number of ER exit sites (ERES) in post-mitotic cells dropped from (3.9 ± 0.6) to (2.7 ± 0.1) eight hours after blocking VSG synthesis. The secretory pathway was still functional in these stalled cells, as monitored using Cathepsin L. Rates of phospholipid and GPI-anchor biosynthesis were relatively unaffected, except for the level of sphingomyelin which increased. However, both ER and Golgi morphology became distorted, with the Golgi cisternae becoming significantly dilated, particularly at the trans-face. Membrane accumulation in these structures is possibly caused by reduced budding of nascent vesicles due to the drastic reduction in the total amount of secretory cargo, i.e. VSG. These data argue that the total flux of secretory cargo impacts upon the biogenesis and maintenance of secretory structures and organelles in T. brucei including the ERES and Golgi.
Citation
Ooi , C-P , Smith , T K , Gluenz , E , Wand , N V , Vaughan , S & Rudenko , G 2018 , ' Blocking variant surface glycoprotein synthesis alters endoplasmic reticulum exit sites/Golgi homeostasis in Trypanosoma brucei ' , Traffic , vol. Early View . https://doi.org/10.1111/tra.12561
Publication
Traffic
Status
Peer reviewed
DOI
https://doi.org/10.1111/tra.12561
ISSN
1600-0854
Type
Journal article
Rights
© 2018 The Authors. Traffic published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Description
This work was supported in part by a Wellcome Trust Senior Research Fellowship award (067441) to T.K.S. G.R. is a Wellcome Senior Fellow in the Basic Biomedical Sciences (095161). This research was funded by the Wellcome Trust.
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  • University of St Andrews Research
URI
http://hdl.handle.net/10023/13102

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