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dc.contributor.authorJoyce, Aoife
dc.contributor.authorIjaz, Umer Z.
dc.contributor.authorNzeteu, Corine
dc.contributor.authorVaughan, Aoife
dc.contributor.authorShirran, Sally L.
dc.contributor.authorBotting, Catherine H.
dc.contributor.authorQuince, Christopher
dc.contributor.authorO’Flaherty, Vincent
dc.contributor.authorAbram, Florence
dc.date.accessioned2018-03-22T11:30:06Z
dc.date.available2018-03-22T11:30:06Z
dc.date.issued2018-03-21
dc.identifier252595944
dc.identifier463cafc2-5bb8-4a86-a506-9448d11f6e04
dc.identifier85044198495
dc.identifier000427947700001
dc.identifier.citationJoyce , A , Ijaz , U Z , Nzeteu , C , Vaughan , A , Shirran , S L , Botting , C H , Quince , C , O’Flaherty , V & Abram , F 2018 , ' Linking microbial community structure and function during the acidified anaerobic digestion of grass ' , Frontiers in Microbiology , vol. 9 . https://doi.org/10.3389/fmicb.2018.00540en
dc.identifier.issn1664-302X
dc.identifier.otherBibtex: urn:f8cb802e609cf556a7a2f6db05362d69
dc.identifier.otherORCID: /0000-0003-3516-3507/work/42954080
dc.identifier.urihttps://hdl.handle.net/10023/12996
dc.descriptionThis research was funded by the Irish Higher Education Authority Program for Research in Third Level Institutions Cycle 5: – PRTLI-5 ESI Ph.D. ENS Program. This work was also supported by the Wellcome Trust (grant number 094476/Z/10/Z for the TripleTOF 5600 mass spectrometer at the University of St Andrews), NERC (grant number NE/L011956/1), and a Royal Irish Academy Mobility Grant.en
dc.description.abstractHarvesting valuable bioproducts from various renewable feedstocks is necessary for the critical development of a sustainable bioeconomy. Anaerobic digestion is a well-established technology for the conversion of wastewater and solid feedstocks to energy with the additional potential for production of process intermediates of high market values (e.g. carboxylates). In recent years, first-generation biofuels typically derived from food crops have been widely utilised as a renewable source of energy. The environmental and socioeconomic limitations of such strategy, however, have led to the development of second-generation biofuels utilising, amongst other feedstocks, lignocellulosic biomass. In this context, the anaerobic digestion of perennial grass holds great promise for the conversion of sustainable renewable feedstock to energy and other process intermediates. The advancement of this technology however, and its implementation for industrial applications, relies on a greater understanding of the microbiome underpinning the process. To this end, microbial communities recovered from replicated anaerobic bioreactors digesting grass were analysed. The bioreactors leachates were not buffered and acidic pH (between 5.5 and 6.3) prevailed at the time of sampling as a result of microbial activities. Community composition and transcriptionally active taxa were examined using 16S rRNA sequencing and microbial functions were investigated using metaproteomics. Bioreactor fraction, i.e. grass or leachate, was found to be the main discriminator of community analysis across the three molecular level of investigation (DNA, RNA and proteins). Six taxa, namely Bacteroidia, Betaproteobacteria, Clostridia, Gammaproteobacteria, Methanomicrobia and Negativicutes accounted for the large majority of the three datasets. The initial stages of grass hydrolysis were carried out by Bacteroidia, Gammaproteobacteria and Negativicutes in the grass biofilms, in addition to Clostridia in the bioreactor leachates. Numerous glycolytic enzymes and carbohydrate transporters were detected throughout the bioreactors in addition to proteins involved in butanol and lactate production. Finally, evidence of the prevalence of stressful conditions within the bioreactors and particularly impacting Clostridia was observed in the metaproteomes. Taken together, this study highlights the functional importance of Clostridia during the anaerobic digestion of grass and thus research avenues allowing members of this taxon to thrive should be explored.
dc.format.extent13
dc.format.extent2351843
dc.language.isoeng
dc.relation.ispartofFrontiers in Microbiologyen
dc.subjectAnaerobic digestionen
dc.subjectCellulosic substrateen
dc.subject16S rRNA profilingen
dc.subjectMetaproteomicsen
dc.subjectBiomolecule co-extractionen
dc.subjectQH301 Biologyen
dc.subjectQR Microbiologyen
dc.subjectDASen
dc.subjectSDG 7 - Affordable and Clean Energyen
dc.subject.lccQH301en
dc.subject.lccQRen
dc.titleLinking microbial community structure and function during the acidified anaerobic digestion of grassen
dc.typeJournal articleen
dc.contributor.sponsorThe Wellcome Trusten
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.contributor.institutionUniversity of St Andrews. School of Chemistryen
dc.contributor.institutionUniversity of St Andrews. EaSTCHEMen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.identifier.doi10.3389/fmicb.2018.00540
dc.description.statusPeer revieweden
dc.identifier.grantnumber094476/Z/10/Zen


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