An unusual flavin-dependent halogenase from the metagenome of the marine sponge Theonella swinhoei WA
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Uncultured bacteria from sponges have been demonstrated to be responsible for the generation of many potent, bioactive natural products including halogenated metabolites.1 The identification of gene clusters from the metagenomes of such bacterial communities enables the discovery of enzymes that mediate new and useful chemistries and allows insight to be gained into the biogenesis of potentially pharmacologically important natural products. Here we report a new pathway to the keramamides (krm); the first functional evidence for the existence of a distinct producer in the Theonella swinhoei WA chemotype is revealed, and a key enzyme on the pathway, a unique flavin dependent halogenase with a broad substrate specificity, and with potential as a useful new biocatalytic tool is described.
Smith , D R M , Uria , A R , Helfrich , E J N , Milbredt , D , van Pée , K-H , Piel , J & Goss , R J M 2017 , ' An unusual flavin-dependent halogenase from the metagenome of the marine sponge Theonella swinhoei WA ' ACS Chemical Biology , vol. 12 , no. 5 , pp. 1281-1287 . DOI: 10.1021/acschembio.6b01115
ACS Chemical Biology
Copyright © 2017 American Chemical Society. This work has been made available online in accordance with the publisher’s policies. This is the author created, accepted version manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at: https://doi.org/10.1021/acschembio.6b01115
DescriptionThe authors thank EU BlueGenics (Seventh Framework Programme, Collaborative project “BlueGenics”, Grant no. 311848 RJMG and JP), the SNF (Grant no.205321_165695 to JP), the Helmut Horten Foundation (JP), and ERAIB (Grant no. 031A338A KHVP and RJMG) for funding.
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