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An integrated perspective on RNA aptamer ligand-recognition models : clearing muddy waters
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dc.contributor.author | McCluskey, K. | |
dc.contributor.author | Penedo, J. Carlos | |
dc.date.accessioned | 2018-02-15T00:33:11Z | |
dc.date.available | 2018-02-15T00:33:11Z | |
dc.date.issued | 2017-03-14 | |
dc.identifier | 249184487 | |
dc.identifier | 921ec42f-9e58-4714-a059-3c3053834ba8 | |
dc.identifier | 85015958366 | |
dc.identifier | 000396148600002 | |
dc.identifier.citation | McCluskey , K & Penedo , J C 2017 , ' An integrated perspective on RNA aptamer ligand-recognition models : clearing muddy waters ' , Physical Chemistry Chemical Physics , vol. 19 , no. 10 , pp. 6921-6932 . https://doi.org/10.1039/C6CP08798A | en |
dc.identifier.issn | 1463-9076 | |
dc.identifier.uri | https://hdl.handle.net/10023/12720 | |
dc.description.abstract | Riboswitches are short RNA motifs that sensitively and selectively bind cognate ligands to modulate gene expression. Like protein receptor-ligand pairs, their binding dynamics are traditionally categorized as following one of two paradigmatic mechanisms: conformational selection and induced fit. In conformational selection, ligand binding stabilizes a particular state already present in the receptor’s dynamic ensemble. In induced fit, ligand-receptor interactions enable the system to overcome the energetic barrier into a previously inaccessible state. In this article, we question whether a polarized division of RNA binding mechanisms truly meets the conceptual needs of the field. We will review the history behind this classification of RNA-ligand interactions, and the way induced fit in particular has been rehabilitated by single-molecule studies of RNA aptamers. We will highlight several recent results from single-molecule experimental studies of riboswitches that reveal gaps or even contradictions between common definitions of the two terms, and we will conclude by proposing a more robust framework that considers the range of RNA behaviors unveiled in recent years as a reality to be described, rather than an increasingly unwieldy set of exceptions to the traditional models. | |
dc.format.extent | 11 | |
dc.format.extent | 1175950 | |
dc.language.iso | eng | |
dc.relation.ispartof | Physical Chemistry Chemical Physics | en |
dc.subject | QD Chemistry | en |
dc.subject | QC Physics | en |
dc.subject | QH426 Genetics | en |
dc.subject.lcc | QD | en |
dc.subject.lcc | QC | en |
dc.subject.lcc | QH426 | en |
dc.title | An integrated perspective on RNA aptamer ligand-recognition models : clearing muddy waters | en |
dc.type | Journal item | en |
dc.contributor.institution | University of St Andrews. School of Physics and Astronomy | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.identifier.doi | https://doi.org/10.1039/C6CP08798A | |
dc.description.status | Peer reviewed | en |
dc.date.embargoedUntil | 2018-02-14 |
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