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dc.contributor.authorGarstang, Myles G.
dc.contributor.authorFerrier, David E. K.
dc.date.accessioned2018-01-10T17:30:05Z
dc.date.available2018-01-10T17:30:05Z
dc.date.issued2018-01
dc.identifier.citationGarstang , M G & Ferrier , D E K 2018 , ' Amphioxus SYCP1 : a case of retrogene replacement and co-option of regulatory elements adjacent to the ParaHox cluster ' , Development Genes and Evolution , vol. 228 , no. 1 , pp. 13-30 . https://doi.org/10.1007/s00427-017-0600-9en
dc.identifier.issn0949-944X
dc.identifier.otherPURE: 251735763
dc.identifier.otherPURE UUID: f70c80bb-9f5e-4d4c-a537-637583514364
dc.identifier.otherScopus: 85039849129
dc.identifier.otherORCID: /0000-0003-3247-6233/work/40266937
dc.identifier.otherWOS: 000424445900002
dc.identifier.urihttp://hdl.handle.net/10023/12458
dc.descriptionMGG was supported by the University of St Andrews School of Biology Biotechnology and Biological Sciences Research Council DTG and the Wellcome Trust ISSF. Work in the authors’ laboratory is also supported by the Leverhulme Trust.en
dc.description.abstractRetrogenes are formed when an mRNA is reverse transcribed and re-inserted into the genome in a location unrelated to the original locus. If this retrocopy inserts into a transcriptionally favourable locus and is able to carry out its original function, it can, in rare cases, lead to retrogene replacement. This involves the original, often multi-exonic, parental copy being lost whilst the newer single-exon retrogene copy ‘replaces’ the role of the ancestral parent gene. One example of this is amphioxus SYCP1, a gene that encodes a protein used in synaptonemal complex formation during meiosis, and which offers the opportunity to examine how a retrogene evolves after the retrogene replacement event. SYCP1 genes exist as large multi-exonic genes in most animals. AmphiSYCP1, however, contains a single coding exon of ~3200bp and has inserted next to the ParaHox cluster of amphioxus, whilst the multi-exonic ancestral parental copy has been lost. Here, we show that AmphiSYCP1 has not only replaced its parental copy, but has evolved additional regulatory function by co- opting a bidirectional promoter from the nearby AmphiCHIC gene. AmphiSYCP1 has also evolved a de novo, multi-exonic 5’untranslated region that displays distinct regulatory states, in the form of two different isoforms, and has evolved novel expression patterns during amphioxus embryogenesis in addition to its ancestral role in meiosis. Absence of ParaHox-like expression of AmphiSYCP1, despite its proximity to the ParaHox cluster, also suggests this gene is not influenced by any potential pan-cluster regulatory mechanisms, which are seemingly restricted to only the ParaHox genes themselves.
dc.format.extent18
dc.language.isoeng
dc.relation.ispartofDevelopment Genes and Evolutionen
dc.rightsCopyright © The Author(s) 2017 This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.en
dc.subjectAmphioxusen
dc.subjectParaHoxen
dc.subjectRetrogene replacementen
dc.subjectSYCPIen
dc.subjectSynaptonemal complexen
dc.subjectCHIC genesen
dc.subjectQH301 Biologyen
dc.subjectQH426 Geneticsen
dc.subjectNDASen
dc.subject.lccQH301en
dc.subject.lccQH426en
dc.titleAmphioxus SYCP1 : a case of retrogene replacement and co-option of regulatory elements adjacent to the ParaHox clusteren
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.contributor.institutionUniversity of St Andrews.Marine Alliance for Science & Technology Scotlanden
dc.contributor.institutionUniversity of St Andrews.Scottish Oceans Instituteen
dc.identifier.doihttps://doi.org/10.1007/s00427-017-0600-9
dc.description.statusPeer revieweden


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