Show simple item record

Files in this item


Item metadata

dc.contributor.authorTang, Anna Ah-San
dc.contributor.authorTiede, Christian
dc.contributor.authorHughes, David J
dc.contributor.authorMcPherson, Michael J
dc.contributor.authorTomlinson, Darren C
dc.identifier.citationTang , A A-S , Tiede , C , Hughes , D J , McPherson , M J & Tomlinson , D C 2017 , ' Isolation of isoform-specific binding proteins (Affimers) by phage display using negative selection ' , Science Signaling , vol. 10 , no. 505 , eaan0868 .
dc.identifier.otherPURE: 251600611
dc.identifier.otherPURE UUID: 31747c3b-a460-448f-86a3-3a44374cfd49
dc.identifier.otherPubMed: 29138294
dc.identifier.otherScopus: 85034228145
dc.identifier.otherORCID: /0000-0002-0090-5710/work/39632387
dc.identifier.otherWOS: 000415705200003
dc.descriptionThis work was funded by the University of Leeds.en
dc.description.abstractSome 30 years after its discovery, phage display remains one of the most widely used methods of in vitro selection. Initially developed to revolutionize the generation of therapeutic antibodies, phage display is now the first choice for screening artificial binding proteins. Artificial binding proteins can be used as reagents to study protein-protein interactions, target posttranslational modifications, and distinguish between homologous proteins. They can also be used as research and affinity reagents, for diagnostic purposes, and as therapeutics. However, the ability to identify isoform-specific reagents remains highly challenging. We describe an adapted phage display protocol using an artificial binding protein (Affimer) for the selection of isoform-selective binding proteins.
dc.relation.ispartofScience Signalingen
dc.rightsCopyright © 2017 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. This work has been made available online in accordance with the publisher’s policies. This is the author created, accepted version manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at
dc.subjectQH301 Biologyen
dc.subjectQD Chemistryen
dc.subjectRM Therapeutics. Pharmacologyen
dc.titleIsolation of isoform-specific binding proteins (Affimers) by phage display using negative selectionen
dc.typeJournal articleen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.description.statusPeer revieweden

This item appears in the following Collection(s)

Show simple item record