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dc.contributor.authorTang, Anna Ah-San
dc.contributor.authorTiede, Christian
dc.contributor.authorHughes, David J
dc.contributor.authorMcPherson, Michael J
dc.contributor.authorTomlinson, Darren C
dc.date.accessioned2017-11-30T16:30:08Z
dc.date.available2017-11-30T16:30:08Z
dc.date.issued2017-11-14
dc.identifier251600611
dc.identifier31747c3b-a460-448f-86a3-3a44374cfd49
dc.identifier29138294
dc.identifier85034228145
dc.identifier000415705200003
dc.identifier.citationTang , A A-S , Tiede , C , Hughes , D J , McPherson , M J & Tomlinson , D C 2017 , ' Isolation of isoform-specific binding proteins (Affimers) by phage display using negative selection ' , Science Signaling , vol. 10 , no. 505 , eaan0868 . https://doi.org/10.1126/scisignal.aan0868en
dc.identifier.issn1945-0877
dc.identifier.otherORCID: /0000-0002-0090-5710/work/39632387
dc.identifier.urihttps://hdl.handle.net/10023/12214
dc.descriptionThis work was funded by the University of Leeds.en
dc.description.abstractSome 30 years after its discovery, phage display remains one of the most widely used methods of in vitro selection. Initially developed to revolutionize the generation of therapeutic antibodies, phage display is now the first choice for screening artificial binding proteins. Artificial binding proteins can be used as reagents to study protein-protein interactions, target posttranslational modifications, and distinguish between homologous proteins. They can also be used as research and affinity reagents, for diagnostic purposes, and as therapeutics. However, the ability to identify isoform-specific reagents remains highly challenging. We describe an adapted phage display protocol using an artificial binding protein (Affimer) for the selection of isoform-selective binding proteins.
dc.format.extent12
dc.format.extent634126
dc.language.isoeng
dc.relation.ispartofScience Signalingen
dc.subjectQH301 Biologyen
dc.subjectQD Chemistryen
dc.subjectRM Therapeutics. Pharmacologyen
dc.subjectT-NDASen
dc.subject.lccQH301en
dc.subject.lccQDen
dc.subject.lccRMen
dc.titleIsolation of isoform-specific binding proteins (Affimers) by phage display using negative selectionen
dc.typeJournal articleen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.identifier.doi10.1126/scisignal.aan0868
dc.description.statusPeer revieweden


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