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dc.contributor.authorOueis, E.
dc.contributor.authorStevenson, H.
dc.contributor.authorJaspars, M.
dc.contributor.authorWestwood, N. J.
dc.contributor.authorNaismith, J. H.
dc.identifier.citationOueis , E , Stevenson , H , Jaspars , M , Westwood , N J & Naismith , J H 2017 , ' Bypassing the proline/thiazoline requirement of the macrocyclase PatG ' , Chemical Communications , vol. 53 , no. 91 , pp. 12274-12277 .
dc.identifier.otherPURE: 251063384
dc.identifier.otherPURE UUID: 79c3a151-e23c-4ab3-ae65-cc796c7c98c1
dc.identifier.otherScopus: 85034048134
dc.identifier.otherORCID: /0000-0003-0630-0138/work/56424177
dc.identifier.otherWOS: 000415061300005
dc.descriptionThis work was supported by the European Research Council (339367), UK Biotechnology and Biological Sciences Research Council (K015508/1), the Wellcome Trust (TripleTOF 5600 mass spectrometer (094476), the MALDI TOF-TOF Analyser (079272AIA), 700 NMR) and the EPSRC UK National Mass Spectrometry Facility at Swansea University. J.H.N. is a Royal Society Wolfson Merit Award Holder and 1000 talent scholar at Sichuan Universityen
dc.description.abstractBiocatalysis is a fast developing field in which an enzyme’s natural capabilities are harnessed or engineered for synthetic chemistry. The enzyme PatG is an extremely promiscuous macrocyclase enzyme tolerating both non-natural amino acids and non-amino acids within the substrate. It does, however, require a proline or thiazoline at the C-terminal position of the core peptide which means the final product must contain this group. Here, we show guided by structural insight we have identified two synthetic routes, triazole and a double cysteine, that circumvent this requirement. With the triazole, we show PatGmac can macrocyclise substrates that do not contain any amino acids in the final product.
dc.relation.ispartofChemical Communicationsen
dc.rights© 2017 the Authors. Open Access Article. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.en
dc.subjectQD Chemistryen
dc.titleBypassing the proline/thiazoline requirement of the macrocyclase PatGen
dc.typeJournal articleen
dc.contributor.sponsorEuropean Research Councilen
dc.contributor.sponsorThe Wellcome Trusten
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Chemistryen
dc.contributor.institutionUniversity of St Andrews. EaSTCHEMen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.description.statusPeer revieweden

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