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dc.contributor.authorOueis, E.
dc.contributor.authorStevenson, H.
dc.contributor.authorJaspars, M.
dc.contributor.authorWestwood, N. J.
dc.contributor.authorNaismith, J. H.
dc.date.accessioned2017-11-22T17:30:06Z
dc.date.available2017-11-22T17:30:06Z
dc.date.issued2017-11-25
dc.identifier.citationOueis , E , Stevenson , H , Jaspars , M , Westwood , N J & Naismith , J H 2017 , ' Bypassing the proline/thiazoline requirement of the macrocyclase PatG ' , Chemical Communications , vol. 53 , no. 91 , pp. 12274-12277 . https://doi.org/10.1039/C7CC06550Gen
dc.identifier.issn1359-7345
dc.identifier.otherPURE: 251063384
dc.identifier.otherPURE UUID: 79c3a151-e23c-4ab3-ae65-cc796c7c98c1
dc.identifier.otherScopus: 85034048134
dc.identifier.otherORCID: /0000-0003-0630-0138/work/56424177
dc.identifier.otherWOS: 000415061300005
dc.identifier.urihttp://hdl.handle.net/10023/12154
dc.descriptionThis work was supported by the European Research Council (339367), UK Biotechnology and Biological Sciences Research Council (K015508/1), the Wellcome Trust (TripleTOF 5600 mass spectrometer (094476), the MALDI TOF-TOF Analyser (079272AIA), 700 NMR) and the EPSRC UK National Mass Spectrometry Facility at Swansea University. J.H.N. is a Royal Society Wolfson Merit Award Holder and 1000 talent scholar at Sichuan Universityen
dc.description.abstractBiocatalysis is a fast developing field in which an enzyme’s natural capabilities are harnessed or engineered for synthetic chemistry. The enzyme PatG is an extremely promiscuous macrocyclase enzyme tolerating both non-natural amino acids and non-amino acids within the substrate. It does, however, require a proline or thiazoline at the C-terminal position of the core peptide which means the final product must contain this group. Here, we show guided by structural insight we have identified two synthetic routes, triazole and a double cysteine, that circumvent this requirement. With the triazole, we show PatGmac can macrocyclise substrates that do not contain any amino acids in the final product.
dc.language.isoeng
dc.relation.ispartofChemical Communicationsen
dc.rights© 2017 the Authors. Open Access Article. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.en
dc.subjectQD Chemistryen
dc.subjectNDASen
dc.subject.lccQDen
dc.titleBypassing the proline/thiazoline requirement of the macrocyclase PatGen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Chemistryen
dc.contributor.institutionUniversity of St Andrews.EaSTCHEMen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1039/C7CC06550G
dc.description.statusPeer revieweden
dc.date.embargoedUntil2017-11-01


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