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The SMN protein is a key regulator of nuclear architecture in differentiating neuroblastoma cells
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dc.contributor.author | Clelland, Allyson K. | |
dc.contributor.author | Kinnear, Nicholas P. | |
dc.contributor.author | Oram, Lisa | |
dc.contributor.author | Burza, Julie | |
dc.contributor.author | Sleeman, Judith Elizabeth | |
dc.date.accessioned | 2010-11-01T16:37:47Z | |
dc.date.available | 2010-11-01T16:37:47Z | |
dc.date.issued | 2009-11 | |
dc.identifier.citation | Clelland , A K , Kinnear , N P , Oram , L , Burza , J & Sleeman , J E 2009 , ' The SMN protein is a key regulator of nuclear architecture in differentiating neuroblastoma cells ' , Traffic , vol. 10 , no. 11 , pp. 1585-1598 . https://doi.org/10.1111/j.1600-0854.2009.00972.x | en |
dc.identifier.issn | 1398-9219 | |
dc.identifier.other | PURE: 3727938 | |
dc.identifier.other | PURE UUID: 3d3e82b4-fc1a-4309-b91b-a4858cb53b1d | |
dc.identifier.other | WOS: 000270665300004 | |
dc.identifier.other | Scopus: 70350635179 | |
dc.identifier.other | ORCID: /0000-0003-0345-6508/work/60426889 | |
dc.identifier.uri | https://hdl.handle.net/10023/1195 | |
dc.description.abstract | The cell nucleus contains two closely related structures, Cajal bodies (CBs) and gems. CBs are the first site of accumulation of newly assembled splicing snRNPs (small nuclear ribonucleoproteins) following their import into the nucleus, before they form their steady-state localization in nuclear splicing speckles. Gems are the nuclear site of accumulation of survival motor neurons (SMNs), an insufficiency of which leads to the inherited neurodegenerative condition, spinal muscular atrophy (SMA). SMN is required in the cytoplasm for the addition of core, Sm, proteins to new snRNPs and is believed to accompany snRNPs to the CB. In most cell lines, gems are indistinguishable from CBs, although the structures are often separate in vivo. The relationship between CBs and gems is not fully understood, but there is evidence that symmetrical dimethylation of arginine residues in the CB protein coilin brings them together in HeLa cells. During neuronal differentiation of the human neuroblastoma cell line SH-SY5Y, CBs and gems increase their colocalization, mimicking changes seen during foetal development. This does not result from alterations in the methylation of coilin, but from increased levels of SMN. Expression of exogenous SMN results in an increased efficiency of snRNP transport to nuclear speckles. This suggests different mechanisms are present in different cell types and in vivo that may be significant for the tissue-specific pathology of SMA. | |
dc.format.extent | 14 | |
dc.language.iso | eng | |
dc.relation.ispartof | Traffic | en |
dc.rights | This is an OnlineOpen article available from http://onlinelibrary.wiley.com | en |
dc.subject | Cajal body | en |
dc.subject | Differentiation | en |
dc.subject | Gem | en |
dc.subject | Nucleus | en |
dc.subject | snRNP maturation | en |
dc.subject | Spinal muscular atrophy | en |
dc.subject | Survival motor neuron | en |
dc.subject | Cajal bodies | en |
dc.subject | Coiled bodies | en |
dc.subject | Motor-neurons | en |
dc.subject | Gene-product | en |
dc.subject | Ribonucleoprotein-particles | en |
dc.subject | Spliceosomal SNRNPS | en |
dc.subject | Fluorescent protein | en |
dc.subject | Splicing SNRNPS | en |
dc.subject | Body formation | en |
dc.subject | QH301 Biology | en |
dc.subject.lcc | QH301 | en |
dc.title | The SMN protein is a key regulator of nuclear architecture in differentiating neuroblastoma cells | en |
dc.type | Journal article | en |
dc.description.version | Publisher PDF | en |
dc.contributor.institution | University of St Andrews. School of Biology | en |
dc.contributor.institution | University of St Andrews. Institute of Behavioural and Neural Sciences | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.identifier.doi | https://doi.org/10.1111/j.1600-0854.2009.00972.x | |
dc.description.status | Peer reviewed | en |
dc.identifier.url | http://www.scopus.com/inward/record.url?scp=70350635179&partnerID=8YFLogxK | en |
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