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Multimode fibre based imaging for optically cleared samples
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dc.contributor.author | Gusachenko, Ivan | |
dc.contributor.author | Nylk, Jonathan | |
dc.contributor.author | Tello, Javier Ananda | |
dc.contributor.author | Dholakia, Kishan | |
dc.date.accessioned | 2017-10-31T10:32:54Z | |
dc.date.available | 2017-10-31T10:32:54Z | |
dc.date.issued | 2017-11-01 | |
dc.identifier.citation | Gusachenko , I , Nylk , J , Tello , J A & Dholakia , K 2017 , ' Multimode fibre based imaging for optically cleared samples ' , Biomedical Optics Express , vol. 8 , no. 11 , pp. 5179-5190 . https://doi.org/10.1364/BOE.8.005179 | en |
dc.identifier.issn | 2156-7085 | |
dc.identifier.other | PURE: 251362936 | |
dc.identifier.other | PURE UUID: d0047bd4-f0f8-42db-b599-78397ab6c5b2 | |
dc.identifier.other | Scopus: 85032834680 | |
dc.identifier.other | ORCID: /0000-0002-2977-4929/work/38331085 | |
dc.identifier.other | ORCID: /0000-0001-6637-2155/work/64034509 | |
dc.identifier.other | WOS: 000414220600031 | |
dc.identifier.uri | https://hdl.handle.net/10023/11958 | |
dc.description | UK Engineering and Physical Sciences Research Council (EPSRC) (EP/P030017/1); European Union’s Horizon 2020 Marie Skłodowska-Curie Actions (MSCA) (707084) | en |
dc.description.abstract | Optical clearing is emerging as a popular approach particularly for studies in neuroscience. However the use of corrosive clearing solutions typically requires sophisticated objectives or extreme care with optical components chosen for single- or multi-photon imaging. In contrast to the use of complex, custom-made microscope objectives, we show that the use of a corrected multimode fibre (MMF) offers a route that is resistant to corrosion, can be used in clearing media, is not constrained by the refractive index of the immersion medium and offers flexible working distances. Using a corrected MMF, we demonstrate fluorescence imaging of beads and stained neuroblastoma cells through optically cleared mouse brain tissue, as well as imaging in an extreme oxidative environment to show the versatility of our approach. Additionally, we perform Raman imaging of polystyrene beads in clearing media to demonstrate that this approach may be used for vibrational spectroscopy of optically cleared samples. | |
dc.language.iso | eng | |
dc.relation.ispartof | Biomedical Optics Express | en |
dc.rights | Copyright the Author(s) 2017. Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI. | en |
dc.subject | QC Physics | en |
dc.subject | RC Internal medicine | en |
dc.subject | T Technology | en |
dc.subject | NDAS | en |
dc.subject.lcc | QC | en |
dc.subject.lcc | RC | en |
dc.subject.lcc | T | en |
dc.title | Multimode fibre based imaging for optically cleared samples | en |
dc.type | Journal article | en |
dc.contributor.sponsor | EPSRC | en |
dc.contributor.sponsor | EPSRC | en |
dc.contributor.sponsor | European Commission | en |
dc.description.version | Publisher PDF | en |
dc.contributor.institution | University of St Andrews. School of Physics and Astronomy | en |
dc.contributor.institution | University of St Andrews. School of Medicine | en |
dc.contributor.institution | University of St Andrews. Cellular Medicine Division | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.identifier.doi | https://doi.org/10.1364/BOE.8.005179 | |
dc.description.status | Peer reviewed | en |
dc.identifier.grantnumber | EP/R004854/1 | en |
dc.identifier.grantnumber | EP/P030017/1 | en |
dc.identifier.grantnumber | 707084 | en |
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