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Influence of a high-fat diet on cardiac iNOS in female rats
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dc.contributor.author | Jovanovic, Aleksandra | |
dc.contributor.author | Sudar-Milovanovic, Emina | |
dc.contributor.author | Obradovic, Milan | |
dc.contributor.author | Pitt, Samantha Jane | |
dc.contributor.author | Stewart, Alan James | |
dc.contributor.author | Zafirovic, Sonja | |
dc.contributor.author | Stanimirovic, Julijana | |
dc.contributor.author | Radak, Djordje | |
dc.contributor.author | Isenovic, Esma R. | |
dc.date.accessioned | 2017-10-25T23:31:52Z | |
dc.date.available | 2017-10-25T23:31:52Z | |
dc.date.issued | 2017 | |
dc.identifier | 246457163 | |
dc.identifier | 135375a6-f857-43aa-b27b-565b80a6dc90 | |
dc.identifier | 27781956 | |
dc.identifier | 85041327847 | |
dc.identifier | 000407476700011 | |
dc.identifier.citation | Jovanovic , A , Sudar-Milovanovic , E , Obradovic , M , Pitt , S J , Stewart , A J , Zafirovic , S , Stanimirovic , J , Radak , D & Isenovic , E R 2017 , ' Influence of a high-fat diet on cardiac iNOS in female rats ' , Current Vascular Pharmacology , vol. 15 , no. 5 , pp. 491-500 . https://doi.org/10.2174/1570161114666161025101303 | en |
dc.identifier.issn | 1570-1611 | |
dc.identifier.other | ORCID: /0000-0003-4580-1840/work/60195778 | |
dc.identifier.other | ORCID: /0000-0003-2257-1595/work/60196222 | |
dc.identifier.uri | https://hdl.handle.net/10023/11921 | |
dc.description.abstract | Overexpression of inducible nitric oxide synthase (iNOS) is a key link between high-fat (HF) diet induced obesity and cardiovascular (CV) disease. Several studies have reported that oestradiol has cardioprotective effects that may be mediated through reduction of iNOS activity/expression. In the present study, female Wistar rats were fed a standard diet or a HF diet (balanced diet for laboratory rats enriched with 42% fat) for 10 weeks. Gene and protein expression of iNOS were measured in heart tissue. HF diet-fed rats exhibited a significant increase in cardiac iNOS mRNA by 695% (p<0.05), iNOS protein level by 248% (p<0.01), without changes in nitrate/nitrite levels. Expression of CD36 protein in plasma membranes was increased by 37% (p<0.05), while the concentration of free fatty acids (FFA) was reduced by 25% (p<0.01) in HF diet-fed rats. Expression of the p50 subunit of nuclear factor-κB (NFκB-p50) in heart lysate was increased by 77% (p<0.01) in HF diet-fed rats. Expression and phosphorylation of protein kinase B (Akt) and extracellular signal-regulated kinases 1/2 (ERK1/2) in control and HF diet-fed rats were also examined. Expression of Akt and ERK1/2 were unchanged between the groups. There was a significant increase in the ratio of phospho-Akt/total Akt but not for phospho-ERK1/2/total ERK1/2/ in HF-fed rats. Estrogen receptor-α levels (by 50%; p<0.05) and serum oestradiol concentrations (by 35%; p<0.05) were examined and shown to be significantly reduced in HF diet-fed rats. Our results revealed that a HF diet led to increased iNOS expression, most likely via a mechanism involving Akt and NFκB-p50 proteins. Decreased levels of oestradiol and ERα protein in the HF-fed group, in combination with increased iNOS levels are consistent with the hypothesis that oestradiol has a cardioprotective effect through its ability to regulate iNOS expression. | |
dc.format.extent | 10 | |
dc.format.extent | 813380 | |
dc.language.iso | eng | |
dc.relation.ispartof | Current Vascular Pharmacology | en |
dc.subject | Cardioprotection | en |
dc.subject | Cardiovascular disease | en |
dc.subject | Oestradiol | en |
dc.subject | Inducible nitric oxide synthase | en |
dc.subject | Obesity | en |
dc.subject | QH426 Genetics | en |
dc.subject | RC Internal medicine | en |
dc.subject | RM Therapeutics. Pharmacology | en |
dc.subject | NDAS | en |
dc.subject | SDG 3 - Good Health and Well-being | en |
dc.subject.lcc | QH426 | en |
dc.subject.lcc | RC | en |
dc.subject.lcc | RM | en |
dc.title | Influence of a high-fat diet on cardiac iNOS in female rats | en |
dc.type | Journal article | en |
dc.contributor.institution | University of St Andrews. School of Medicine | en |
dc.contributor.institution | University of St Andrews. Institute of Behavioural and Neural Sciences | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.contributor.institution | University of St Andrews. Cellular Medicine Division | en |
dc.identifier.doi | 10.2174/1570161114666161025101303 | |
dc.description.status | Peer reviewed | en |
dc.date.embargoedUntil | 2017-10-25 |
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