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dc.contributor.authorLancefield, Christopher S.
dc.contributor.authorRashid, Goran M. M.
dc.contributor.authorBouxin, Florent
dc.contributor.authorWasak, Agata
dc.contributor.authorTu, Wei-Chien
dc.contributor.authorHallett, Jason P.
dc.contributor.authorZein, Sharif
dc.contributor.authorRodríguez, Jaime
dc.contributor.authorJackson, S. David
dc.contributor.authorWestwood, Nicholas J.
dc.contributor.authorBugg, Timothy D. H.
dc.identifier.citationLancefield , C S , Rashid , G M M , Bouxin , F , Wasak , A , Tu , W-C , Hallett , J P , Zein , S , Rodríguez , J , Jackson , S D , Westwood , N J & Bugg , T D H 2016 , ' Investigation of the chemocatalytic and biocatalytic valorization of a range of different lignin preparations : the importance of β-O-4 content ' , ACS Sustainable Chemistry & Engineering , vol. 4 , no. 12 , pp. 6921-6930 .
dc.identifier.otherPURE: 246489876
dc.identifier.otherPURE UUID: 175add4e-1e12-461d-a7b6-cf84281f753f
dc.identifier.otherScopus: 85001967835
dc.identifier.otherORCID: /0000-0003-0630-0138/work/56424188
dc.identifier.otherWOS: 000389497900075
dc.descriptionThis project was supported by a grant from the Lignocellulosic Biorefinery Network, a BBSRC-funded network in Biotechnology and Bioenergy.en
dc.description.abstractA set of seven different lignin preparations was generated from a range of organosolv (acidic, alkaline, ammonia-treated and dioxane-based), ionic liquid, autohydrolysis and Kraft pretreatments of lignocelluloses. Each lignin was characterised by 2D HSQC NMR spectroscopy, showing significant variability in the β-O-4 content of the different lignin samples. Each lignin was then valorised using three biocatalytic methods (microbial biotransformation with Rhodococcus jostii RHA045, treatment with Pseudomonas fluorescens Dyp1B or Sphingobacterium sp. T2 manganese superoxide dismutase) and two chemocatalytic methods (catalytic hydrogenation using Pt/alumina catalyst, DDQ benzylic oxidation/Zn reduction). Highest product yields for DDQ/Zn valorisation were observed from poplar ammonia percolation-organosolv lignin, which had the highest β-O-4 content of the investigated lignins and also gave the highest yield of syringaldehyde (243 mg L-1) when using R. jostii RHA045, and the most enzymatic products using P. fluorescens Dyp1B. The highest product yield from the Pt/alumina hydrogenation was observed using oak dioxasolv lignin, which also had a high β-O-4 content. In general, highest product yields for both chemocatalytic and biocatalytic valorisation methods were obtained from preparations that showed highest β-O-4 content, while variable yields were obtained with preparations containing intermediate β-O-4 content, and little or no product was obtained with preparations containing low β-O-4 content.
dc.relation.ispartofACS Sustainable Chemistry & Engineeringen
dc.rightsCopyright © 2016 American Chemical Society. This work is made available online in accordance with the publisher’s policies. This is the author created, accepted version manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at
dc.subjectCatalytic hydrogenationen
dc.subjectDyp peroxidaseen
dc.subjectLignin valorizationen
dc.subjectMicrobial biotransformationen
dc.subjectRhodococcus jostii RHA1en
dc.subjectSuperoxide dismutaseen
dc.subjectQD Chemistryen
dc.titleInvestigation of the chemocatalytic and biocatalytic valorization of a range of different lignin preparations : the importance of β-O-4 contenten
dc.typeJournal articleen
dc.contributor.institutionUniversity of St Andrews. School of Chemistryen
dc.contributor.institutionUniversity of St Andrews. EaSTCHEMen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.description.statusPeer revieweden

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