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dc.contributor.authorBaron, Vincent
dc.contributor.authorChen, Mingzhou
dc.contributor.authorClark, Simon O.
dc.contributor.authorWilliams, Ann
dc.contributor.authorHammond, Robert James Hunter
dc.contributor.authorDholakia, Kishan
dc.contributor.authorGillespie, Stephen Henry
dc.date.accessioned2017-09-01T16:30:11Z
dc.date.available2017-09-01T16:30:11Z
dc.date.issued2017-08-29
dc.identifier250675702
dc.identifier96feae3f-f715-41a7-bbaa-3938ef253693
dc.identifier85028516207
dc.identifier000408538100086
dc.identifier.citationBaron , V , Chen , M , Clark , S O , Williams , A , Hammond , R J H , Dholakia , K & Gillespie , S H 2017 , ' Label-free optical vibrational spectroscopy to detect the metabolic state of M. tuberculosis cells at the site of disease ' , Scientific Reports , vol. 7 , 9844 . https://doi.org/10.1038/s41598-017-10234-zen
dc.identifier.issn2045-2322
dc.identifier.otherORCID: /0000-0001-6537-7712/work/39477842
dc.identifier.otherORCID: /0000-0002-6190-5167/work/47136395
dc.identifier.urihttps://hdl.handle.net/10023/11594
dc.descriptionThis work was supported by the PreDiCT-TB consortium [IMI Joint undertaking grant agreement number 115337, resources of which are composed of financial contribution from the European Union’s Seventh Framework Programme (FP7/2007-2013) and EFPIA companies’ in kind contribution (www.imi.europa.eu)]. M.C. and K.D. thank the UK Engineering and Physical Sciences Research Council (Grant code EP/J01771X/1) and a European Union FAMOS project (FP7 ICT, 317744) for funding. K.D. acknowledges support from a Royal Society Leverhulme Trust Senior Fellowship and the loan of a laser from M Squared Lasers. This work was supported by the Department of Health, UK. The views expressed in this publication are those of the authors and not necessarily those of the Department of Health.en
dc.description.abstractTuberculosis relapse is a barrier to shorter treatment. It is thought that lipid rich cells, phenotypically resistant to antibiotics, may play a major role. Most studies investigating relapse use sputum samples although tissue bacteria may play an important role. We developed a nondestructive, label-free technique combining wavelength modulated Raman (WMR) spectroscopy and fluorescence detection (Nile Red staining) to interrogate Mycobacterium tuberculosis cell state. This approach could differentiate single “dormant” (lipid rich, LR) and “non-dormant” (lipid poor, LP) cells with high sensitivity and specificity. We applied this to experimentally infected guinea pig lung sections and were able to distinguish both cell types showing that the LR phenotype dominates in infected tissue. Both in-vitro and ex-vivo spectra correlated well, showing for the first time that Mycobacterium tuberculosis, likely to be phenotypically resistant to antibiotics, are present in large numbers in tissue. This is an important step in understanding the pathology of relapse supporting the idea that they may be caused by M. tuberculosis cells with lipid inclusions.
dc.format.extent9
dc.format.extent1845129
dc.language.isoeng
dc.relation.ispartofScientific Reportsen
dc.subjectQC Physicsen
dc.subjectQR Microbiologyen
dc.subjectT Technologyen
dc.subjectDASen
dc.subjectBDCen
dc.subjectSDG 3 - Good Health and Well-beingen
dc.subject.lccQCen
dc.subject.lccQRen
dc.subject.lccTen
dc.titleLabel-free optical vibrational spectroscopy to detect the metabolic state of M. tuberculosis cells at the site of diseaseen
dc.typeJournal articleen
dc.contributor.sponsorEPSRCen
dc.contributor.sponsorEuropean Commissionen
dc.contributor.sponsorEuropean Commissionen
dc.contributor.institutionUniversity of St Andrews. School of Physics and Astronomyen
dc.contributor.institutionUniversity of St Andrews. School of Medicineen
dc.contributor.institutionUniversity of St Andrews. Global Health Implementation Groupen
dc.contributor.institutionUniversity of St Andrews. Gillespie Groupen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews. Infection Groupen
dc.contributor.institutionUniversity of St Andrews. Infection and Global Health Divisionen
dc.identifier.doi10.1038/s41598-017-10234-z
dc.description.statusPeer revieweden
dc.identifier.grantnumberEP/J01771X/1en
dc.identifier.grantnumber317744en
dc.identifier.grantnumberen


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