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dc.contributor.authorGiannoulis, Angeliki
dc.contributor.authorOranges, Maria
dc.contributor.authorBode, Bela E.
dc.date.accessioned2017-08-29T09:30:07Z
dc.date.available2017-08-29T09:30:07Z
dc.date.issued2017-09-06
dc.identifier250364687
dc.identifier89c4e647-d52b-4ed5-9ae7-0d64c1bb90b1
dc.identifier85026520268
dc.identifier000409334300003
dc.identifier.citationGiannoulis , A , Oranges , M & Bode , B E 2017 , ' Monitoring complex formation by relaxation-induced pulse electron paramagnetic resonance distance measurements ' , ChemPhysChem , vol. 18 , no. 17 , pp. 2318-2321 . https://doi.org/10.1002/cphc.201700666en
dc.identifier.issn1439-4235
dc.identifier.otherORCID: /0000-0002-3384-271X/work/36590460
dc.identifier.urihttps://hdl.handle.net/10023/11560
dc.descriptionFunding: EPSRC DTC and Wellcome (099149/Z/12/Z).en
dc.description.abstractBiomolecular complexes are often multimers fueling the demand for methods that allow unraveling their composition and geometric arrangement. Pulse electron paramagnetic resonance (EPR) spectroscopy is increasingly applied for retrieving geometric information on the nanometer scale. The emerging RIDME (relaxation-induced dipolar modulation enhancement) technique offers improved sensitivity in distance experiments involving metal centers (e.g. on metalloproteins or proteins labelled with metal ions). Here, a mixture of a spin labelled ligand with increasing amounts of paramagnetic CuII ions allowed accurate quantification of ligand-metal binding in the model complex formed. The distance measurement was highly accurate and critical aspects for identifying multimerization could be identified. The potential to quantify binding in addition to the high-precision distance measurement will further increase the scope of EPR applications.
dc.format.extent5
dc.format.extent610676
dc.language.isoeng
dc.relation.ispartofChemPhysChemen
dc.subjectComplexesen
dc.subjectDistance measurementsen
dc.subjectEPR spectroscopyen
dc.subjectMetalloproteinsen
dc.subjectMultimersen
dc.subjectQD Chemistryen
dc.subjectQH301 Biologyen
dc.subjectDASen
dc.subject.lccQDen
dc.subject.lccQH301en
dc.titleMonitoring complex formation by relaxation-induced pulse electron paramagnetic resonance distance measurementsen
dc.typeJournal articleen
dc.contributor.sponsorThe Wellcome Trusten
dc.contributor.sponsorEuropean Commissionen
dc.contributor.sponsorThe Royal Societyen
dc.contributor.institutionUniversity of St Andrews. School of Chemistryen
dc.contributor.institutionUniversity of St Andrews. Centre of Magnetic Resonanceen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews. EaSTCHEMen
dc.identifier.doi10.1002/cphc.201700666
dc.description.statusPeer revieweden
dc.date.embargoedUntil2017-08-01
dc.identifier.grantnumber099149/Z/12/Zen
dc.identifier.grantnumberen
dc.identifier.grantnumberRG140723en


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