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Monitoring complex formation by relaxation-induced pulse electron paramagnetic resonance distance measurements
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dc.contributor.author | Giannoulis, Angeliki | |
dc.contributor.author | Oranges, Maria | |
dc.contributor.author | Bode, Bela E. | |
dc.date.accessioned | 2017-08-29T09:30:07Z | |
dc.date.available | 2017-08-29T09:30:07Z | |
dc.date.issued | 2017-09-06 | |
dc.identifier | 250364687 | |
dc.identifier | 89c4e647-d52b-4ed5-9ae7-0d64c1bb90b1 | |
dc.identifier | 85026520268 | |
dc.identifier | 000409334300003 | |
dc.identifier.citation | Giannoulis , A , Oranges , M & Bode , B E 2017 , ' Monitoring complex formation by relaxation-induced pulse electron paramagnetic resonance distance measurements ' , ChemPhysChem , vol. 18 , no. 17 , pp. 2318-2321 . https://doi.org/10.1002/cphc.201700666 | en |
dc.identifier.issn | 1439-4235 | |
dc.identifier.other | ORCID: /0000-0002-3384-271X/work/36590460 | |
dc.identifier.uri | https://hdl.handle.net/10023/11560 | |
dc.description | Funding: EPSRC DTC and Wellcome (099149/Z/12/Z). | en |
dc.description.abstract | Biomolecular complexes are often multimers fueling the demand for methods that allow unraveling their composition and geometric arrangement. Pulse electron paramagnetic resonance (EPR) spectroscopy is increasingly applied for retrieving geometric information on the nanometer scale. The emerging RIDME (relaxation-induced dipolar modulation enhancement) technique offers improved sensitivity in distance experiments involving metal centers (e.g. on metalloproteins or proteins labelled with metal ions). Here, a mixture of a spin labelled ligand with increasing amounts of paramagnetic CuII ions allowed accurate quantification of ligand-metal binding in the model complex formed. The distance measurement was highly accurate and critical aspects for identifying multimerization could be identified. The potential to quantify binding in addition to the high-precision distance measurement will further increase the scope of EPR applications. | |
dc.format.extent | 5 | |
dc.format.extent | 610676 | |
dc.language.iso | eng | |
dc.relation.ispartof | ChemPhysChem | en |
dc.subject | Complexes | en |
dc.subject | Distance measurements | en |
dc.subject | EPR spectroscopy | en |
dc.subject | Metalloproteins | en |
dc.subject | Multimers | en |
dc.subject | QD Chemistry | en |
dc.subject | QH301 Biology | en |
dc.subject | DAS | en |
dc.subject.lcc | QD | en |
dc.subject.lcc | QH301 | en |
dc.title | Monitoring complex formation by relaxation-induced pulse electron paramagnetic resonance distance measurements | en |
dc.type | Journal article | en |
dc.contributor.sponsor | The Wellcome Trust | en |
dc.contributor.sponsor | European Commission | en |
dc.contributor.sponsor | The Royal Society | en |
dc.contributor.institution | University of St Andrews. School of Chemistry | en |
dc.contributor.institution | University of St Andrews. Centre of Magnetic Resonance | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.contributor.institution | University of St Andrews. EaSTCHEM | en |
dc.identifier.doi | https://doi.org/10.1002/cphc.201700666 | |
dc.description.status | Peer reviewed | en |
dc.date.embargoedUntil | 2017-08-01 | |
dc.identifier.grantnumber | 099149/Z/12/Z | en |
dc.identifier.grantnumber | en | |
dc.identifier.grantnumber | RG140723 | en |
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