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dc.contributor.authorSharma, Sunil V.
dc.contributor.authorTong, Xiaoxue
dc.contributor.authorPubill-Ulldemolins, Cristina
dc.contributor.authorCartmell, Christopher
dc.contributor.authorBogosyan, Emma J. A.
dc.contributor.authorRackham, Emma J.
dc.contributor.authorMarelli, Enrico
dc.contributor.authorHamed, Refaat B.
dc.contributor.authorGoss, Rebecca J.M.
dc.identifier.citationSharma , S V , Tong , X , Pubill-Ulldemolins , C , Cartmell , C , Bogosyan , E J A , Rackham , E J , Marelli , E , Hamed , R B & Goss , R J M 2017 , ' Living GenoChemetics by hyphenating synthetic biology and synthetic chemistry in vivo ' , Nature Communications , vol. 8 , 229 .
dc.identifier.otherPURE: 250890459
dc.identifier.otherPURE UUID: cbc03331-4d47-4811-912a-3cfd1b26bded
dc.identifier.otherScopus: 85027232303
dc.identifier.otherWOS: 000407198800018
dc.descriptionWe thank the European Research Council under the European Union’s Seventh Framework Programme (FP7/2007–2013/ERC consolidator grant GCGXC grant agreement no 614779) and ERAIB (Grant no. 031A338A) and H2020-MSCA-IF-2014 Grant no. 659399 for generous financial support.en
dc.description.abstractMarrying synthetic biology with synthetic chemistry provides a powerful approach toward natural product diversification, combining the best of both worlds: expediency and synthetic capability of biogenic pathways and chemical diversity enabled by organic synthesis. Biosynthetic pathway engineering can be employed to insert a chemically orthogonal tag into a complex natural scaffold affording the possibility of site-selective modification without employing protecting group strategies. Here we show that, by installing a sufficiently reactive handle (e.g., a C-Br bond) and developing compatible mild aqueous chemistries, synchronous biosynthesis of the tagged metabolite and its subsequent chemical modification in living culture can be achieved. This approach can potentially enable many new applications: for example, assay of directed evolution of enzymes catalyzing halo-metabolite biosynthesis in living cells or generating and following the fate of tagged metabolites and biomolecules in living systems. We report synthetic biological access to new-to-nature bromo-metabolites and the concomitant biorthogonal cross-coupling of halo-metabolites in living cultures.
dc.relation.ispartofNature Communicationsen
dc.rights© The Author(s) 2017. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit
dc.subjectQD Chemistryen
dc.subjectQH301 Biologyen
dc.subjectBiochemistry, Genetics and Molecular Biology(all)en
dc.subjectPhysics and Astronomy(all)en
dc.titleLiving GenoChemetics by hyphenating synthetic biology and synthetic chemistry in vivoen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Chemistryen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews.EaSTCHEMen
dc.description.statusPeer revieweden

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