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Authentication and characterisation of a new oesophageal adenocarcinoma cell line : MFD-1
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dc.contributor.author | Garcia, Edwin | |
dc.contributor.author | Hayden, Annette | |
dc.contributor.author | Birts, Charles | |
dc.contributor.author | Britton, Edward | |
dc.contributor.author | Cowie, Andrew | |
dc.contributor.author | Pickard, Karen | |
dc.contributor.author | Mellone, Massimiliano | |
dc.contributor.author | Choh, Clarisa | |
dc.contributor.author | Derouet, Mathieu | |
dc.contributor.author | Duriez, Patrick | |
dc.contributor.author | Noble, Fergus | |
dc.contributor.author | White, Michael J. | |
dc.contributor.author | Primrose, John N. | |
dc.contributor.author | Strefford, Jonathan C. | |
dc.contributor.author | Rose-Zerilli, Matthew | |
dc.contributor.author | Thomas, Gareth J. | |
dc.contributor.author | Ang, Yeng | |
dc.contributor.author | Sharrocks, Andrew D. | |
dc.contributor.author | Fitzgerald, Rebecca C. | |
dc.contributor.author | Underwood, Timothy J. | |
dc.contributor.author | OCCAMS Consortium | |
dc.date.accessioned | 2017-08-16T10:30:11Z | |
dc.date.available | 2017-08-16T10:30:11Z | |
dc.date.issued | 2016-09-07 | |
dc.identifier | 250847629 | |
dc.identifier | 4dece571-59c6-40b8-9b54-65f70b64b806 | |
dc.identifier | 84987680020 | |
dc.identifier.citation | Garcia , E , Hayden , A , Birts , C , Britton , E , Cowie , A , Pickard , K , Mellone , M , Choh , C , Derouet , M , Duriez , P , Noble , F , White , M J , Primrose , J N , Strefford , J C , Rose-Zerilli , M , Thomas , G J , Ang , Y , Sharrocks , A D , Fitzgerald , R C , Underwood , T J & OCCAMS Consortium 2016 , ' Authentication and characterisation of a new oesophageal adenocarcinoma cell line : MFD-1 ' , Scientific Reports , vol. 6 , 32417 . https://doi.org/10.1038/srep32417 | en |
dc.identifier.issn | 2045-2322 | |
dc.identifier.other | ORCID: /0000-0002-7876-7338/work/36106404 | |
dc.identifier.uri | https://hdl.handle.net/10023/11487 | |
dc.description.abstract | New biological tools are required to understand the functional significance of genetic events revealed by whole genome sequencing (WGS) studies in oesophageal adenocarcinoma (OAC). The MFD-1 cell line was isolated from a 55-year-old male with OAC without recombinant-DNA transformation. Somatic genetic variations from MFD-1, tumour, normal oesophagus, and leucocytes were analysed with SNP6. WGS was performed in tumour and leucocytes. RNAseq was performed in MFD-1, and two classic OAC cell lines FLO1 and OE33. Transposase-accessible chromatin sequencing (ATAC-seq) was performed in MFD-1, OE33, and non-neoplastic HET1A cells. Functional studies were performed. MFD-1 had a high SNP genotype concordance with matched germline/tumour. Parental tumour and MFD-1 carried four somatically acquired mutations in three recurrent mutated genes in OAC: TP53, ABCB1 and SEMA5A, not present in FLO-1 or OE33. MFD-1 displayed high expression of epithelial and glandular markers and a unique fingerprint of open chromatin. MFD-1 was tumorigenic in SCID mouse and proliferative and invasive in 3D cultures. The clinical utility of whole genome sequencing projects will be delivered using accurate model systems to develop molecular-phenotype therapeutics. We have described the first such system to arise from the oesophageal International Cancer Genome Consortium project. | |
dc.format.extent | 12 | |
dc.format.extent | 2003561 | |
dc.language.iso | eng | |
dc.relation.ispartof | Scientific Reports | en |
dc.subject | QH301 Biology | en |
dc.subject | QH426 Genetics | en |
dc.subject | RC0254 Neoplasms. Tumors. Oncology (including Cancer) | en |
dc.subject | NDAS | en |
dc.subject | SDG 3 - Good Health and Well-being | en |
dc.subject.lcc | QH301 | en |
dc.subject.lcc | QH426 | en |
dc.subject.lcc | RC0254 | en |
dc.title | Authentication and characterisation of a new oesophageal adenocarcinoma cell line : MFD-1 | en |
dc.type | Journal article | en |
dc.contributor.institution | University of St Andrews. School of Medicine | en |
dc.contributor.institution | University of St Andrews. Statistics | en |
dc.identifier.doi | 10.1038/srep32417 | |
dc.description.status | Peer reviewed | en |
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