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dc.contributor.authorWarner, Emily F.
dc.contributor.authorSmith, Michael J.
dc.contributor.authorZhang, Qingzhi
dc.contributor.authorRaheem, K. Saki
dc.contributor.authorO'Hagan, David
dc.contributor.authorO'Connell, Maria A.
dc.contributor.authorKay, Colin D.
dc.date.accessioned2017-06-19T15:30:09Z
dc.date.available2017-06-19T15:30:09Z
dc.date.issued2017-09
dc.identifier.citationWarner , E F , Smith , M J , Zhang , Q , Raheem , K S , O'Hagan , D , O'Connell , M A & Kay , C D 2017 , ' Signatures of anthocyanin metabolites identified in humans inhibit biomarkers of vascular inflammation in human endothelial cells ' , Molecular Nutrition and Food Research , vol. 61 , no. 9 , 1700053 . https://doi.org/10.1002/mnfr.201700053en
dc.identifier.issn1613-4133
dc.identifier.otherPURE: 249911193
dc.identifier.otherPURE UUID: 695a4d21-8cf5-4bcf-b2c3-43a5a885a100
dc.identifier.otherBibtex: urn:f98214ffe339dd91fb4060266e68d22a
dc.identifier.otherScopus: 85020413571
dc.identifier.urihttp://hdl.handle.net/10023/11027
dc.descriptionThis study was supported by funding from the UK Biotechnology and Biological Sciences Research Council Diet and Health Research Industry Club (BBSRC-DRINC; BB/H004963/1, BB/H00503X/1 and BB/H004726).en
dc.description.abstractScope :  The physiological relevance of contemporary cell culture studies is often perplexing, given the use of unmetabolized phytochemicals at supraphysiological concentrations. We investigated the activity of physiologically relevant anthocyanin metabolite signatures, derived from a previous pharmacokinetics study of 500 mg 13C5-cyanidin-3-glucoside in 8 healthy participants, on soluble vascular adhesion molecule-1 (VCAM-1) and interleukin-6 (IL-6) in human endothelial cells. Methods and results:   Signatures of peak metabolites (previously identified at 1, 6 and 24 h post-bolus) were reproduced using pure standards and effects were investigated across concentrations ten-fold lower and higher than observed mean (<5 μM) serum levels. Tumor necrosis factor-α (TNF-α)-stimulated VCAM-1 was reduced in response to all treatments, with maximal effects observed for the 6 h and 24 h profiles. Profiles tested at ten-fold below mean serum concentrations (0.19-0.44 μM) remained active. IL-6 was reduced in response to 1, 6 and 24 h profiles, with maximal effects observed for 6 h and 24 h profiles at concentrations above 2 μM. Protein responses were reflected by reductions in VCAM-1 and IL-6 mRNA, however there was no effect on phosphorylated NFκB-p65 expression. Conclusion:  Signatures of anthocyanin metabolites following dietary consumption reduce VCAM-1 and IL-6 production, providing evidence of physiologically relevant biological activity.
dc.language.isoeng
dc.relation.ispartofMolecular Nutrition and Food Researchen
dc.rights© 2017 The Authors. Molecular Nutrition & Food Research published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.en
dc.subjectAdhesionen
dc.subjectAnthocyaninen
dc.subjectInflammationen
dc.subjectMetabolismen
dc.subjectQD Chemistryen
dc.subjectNDASen
dc.subject.lccQDen
dc.titleSignatures of anthocyanin metabolites identified in humans inhibit biomarkers of vascular inflammation in human endothelial cellsen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Chemistryen
dc.contributor.institutionUniversity of St Andrews.EaSTCHEMen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1002/mnfr.201700053
dc.description.statusPeer revieweden
dc.date.embargoedUntil2017-06-09


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