Show simple item record

Files in this item


Item metadata

dc.contributor.authorKubasiewicz, L. M.
dc.contributor.authorMinderman, Jeroen
dc.contributor.authorWoodall, L. C.
dc.contributor.authorQuine, C. P.
dc.contributor.authorCoope, R.
dc.contributor.authorPark, Kirsty J.
dc.identifier.citationKubasiewicz , L M , Minderman , J , Woodall , L C , Quine , C P , Coope , R & Park , K J 2016 , ' Fur and faeces : an experimental assessment of non-invasive DNA sampling for the European pine marten ' , Mammal Research , vol. 61 , no. 4 , pp. 299-307 .
dc.identifier.otherPURE: 242895389
dc.identifier.otherPURE UUID: 7f53bf4e-01e5-42d0-b97e-182882356b7a
dc.identifier.otherScopus: 84988602770
dc.identifier.otherWOS: 000384589700001
dc.descriptionThis project was funded by the University of Stirling, Forestry Commission, Forest Research, the Royal Society of the Protection of Birds and Scottish Natural Heritage.en
dc.description.abstractNon-invasive genetic sampling using materials such as faeces or hair can be used to monitor wildlife populations, although DNA quality is often poor. Improving sampling efficiency and minimising factors that reduce DNA quality are therefore critical. After a severe decline, the European pine marten, Martes martes, has reclaimed much of its former range in Scotland, UK. Recording this rapid range expansion requires developing techniques for accurate monitoring, but this is hampered by the species’ elusive behaviour. We tested two sampling methods, hair collected from hair tubes and faeces (scat) collected along tracks, to assess the effects of key environmental and sampling variables on DNA quality and sampling efficiency. For hair, we tested the influence of hair tube location (distance from forest tracks) on collection rate and sex ratio of animals successfully sampled. For scats, we assessed the effect of time since defecation (1 to 16 days) on genotyping error rates and success under two contrasting environmental conditions (exposed to rainfall or sheltered). We found no bias in the collection rate or sex ratio of animals detected by hair samples with differing proximity to forest tracks. DNA amplification failure for scats exposed to rainfall increased from 28 to 65 % over the 16-day experimental period. During periods of low rainfall, the length of collection sessions could therefore be extended to increase sample number without risk of DNA degradation. Lack of bias in hair collection rates with proximity to forest tracks provides justification for tube placement close to tracks, as this reduces survey effort. These findings provide guidance for the development of efficient and cost-effective non-invasive sampling of Scottish pine martens.
dc.relation.ispartofMammal Researchen
dc.rights© 2016, Crown Copyright. This work is made available online in accordance with the publisher’s policies. This is the author created, accepted version manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at /
dc.subjectNon-invasive geneticsen
dc.subjectElusive speciesen
dc.subjectDNA degradationen
dc.subjectMartes martesen
dc.subjectAllelic dropouten
dc.subjectFalse allelesen
dc.subjectQH301 Biologyen
dc.subjectQH426 Geneticsen
dc.subjectQL Zoologyen
dc.titleFur and faeces : an experimental assessment of non-invasive DNA sampling for the European pine martenen
dc.typeJournal articleen
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.description.statusPeer revieweden

This item appears in the following Collection(s)

Show simple item record