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dc.contributor.authorTilston-Lunel, Andrew Martin
dc.contributor.authorHaley, Kathryn
dc.contributor.authorSchlecht, Nicolas
dc.contributor.authorWang, Yanhua
dc.contributor.authorChatterton, Abigail Louise Dougal
dc.contributor.authorMoleirinho, Susana Luisa
dc.contributor.authorWatson, Ailsa
dc.contributor.authorHundal, Harinder
dc.contributor.authorPrystowsky, Michael
dc.contributor.authorGunn-Moore, Frank J.
dc.contributor.authorReynolds, Paul Andrew
dc.date.accessioned2017-04-18T23:33:49Z
dc.date.available2017-04-18T23:33:49Z
dc.date.issued2016-10-07
dc.identifier.citationTilston-Lunel , A M , Haley , K , Schlecht , N , Wang , Y , Chatterton , A L D , Moleirinho , S L , Watson , A , Hundal , H , Prystowsky , M , Gunn-Moore , F J & Reynolds , P A 2016 , ' Crumbs 3b promotes tight junctions in an ezrin-dependent manner in mammalian cells ' , Journal of Molecular Cell Biology , vol. 8 , no. 5 , pp. 439-455 . https://doi.org/10.1093/jmcb/MJW020en
dc.identifier.issn1674-2788
dc.identifier.otherPURE: 240593744
dc.identifier.otherPURE UUID: 796faea7-067b-420d-abe3-4cbf997c0640
dc.identifier.otherScopus: 84992315629
dc.identifier.otherORCID: /0000-0001-8738-1245/work/34034577
dc.identifier.otherORCID: /0000-0003-3422-3387/work/34730413
dc.identifier.otherWOS: 000386455300007
dc.identifier.urihttp://hdl.handle.net/10023/10628
dc.descriptionAMT-L is supported by the School of Biology, University of St Andrews. AMT-L, PAR and FJGM were funded by the Anonymous Trust, University of St Andrews. PAR is supported by the Melville Trust for the Care and Cure of Cancer. The mass spectrometry work was supported by the Wellcome Trust [grant number 094476/Z/10/Z], which funded the purchase of the TripleTOF 5600 mass spectrometer at the BSRC Mass Spectrometry and Proteomics Facility, University of St Andrews. The clinical study was supported by the Department of Pathology, Albert Einstein College of Medicine/ Montefiore Medical Center.en
dc.description.abstractCrumbs3 (CRB3) is a component of epithelial junctions that has been implicated in apical-basal polarity, apical identity, apical stability, cell adhesion and cell growth. CRB3 undergoes alternative splicing to yield two variants: CRB3a and CRB3b. Here, we describe novel data demonstrating that as with previous studies on CRB3a, CRB3b also promotes the formation of tight junctions. However, significantly we demonstrate that the 4.1-ezrin-radixin-moesin (FERM) binding motif (FBM) of CRB3b is required for CRB3b functionality and that ezrin binds to the FBM of CRB3b. Furthermore, we show that ezrin contributes to CRB3b functionality and the correct distribution of tight junction proteins. We demonstrate that both CRB3 isoforms are required for the production of functionally mature tight junctions and also the localization of ezrin to the plasma membrane. Finally, we demonstrate that reduced CRB3b expression in head and neck squamous cell carcinoma (HNSCC) correlates with cytoplasmic ezrin, a biomarker for aggressive disease, and show evidence that whilst CRB3a expression has no effect, low CRB3b and high cytoplasmic ezrin expression combined may be prognostic for HNSCC.
dc.language.isoeng
dc.relation.ispartofJournal of Molecular Cell Biologyen
dc.rights© The Author (2016). Published by Oxford University Press on behalf of Journal of Molecular Cell Biology, IBCB, SIBS, CAS. This work is made available online in accordance with the publisher’s policies. This is the author created, accepted version manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at https://dx.doi.org/10.1093/jmcb/mjw020en
dc.subjectCRB3en
dc.subjectCrumbsen
dc.subjectFERM proteinsen
dc.subjectFBMen
dc.subjectTight junctionsen
dc.subjectEzrinen
dc.subjectQH301 Biologyen
dc.subjectNDASen
dc.subjectBDCen
dc.subjectR2Cen
dc.subject.lccQH301en
dc.titleCrumbs 3b promotes tight junctions in an ezrin-dependent manner in mammalian cellsen
dc.typeJournal articleen
dc.description.versionPostprinten
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.contributor.institutionUniversity of St Andrews.School of Medicineen
dc.contributor.institutionUniversity of St Andrews.Institute of Behavioural and Neural Sciencesen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1093/jmcb/MJW020
dc.description.statusPeer revieweden
dc.date.embargoedUntil2017-04-18


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