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dc.contributor.authorPascoal, Sonia Christina Marques
dc.contributor.authorLiu, Xuan
dc.contributor.authorLy, Tony
dc.contributor.authorFang, Yongxiang
dc.contributor.authorRockliffe, Nichola
dc.contributor.authorPaterson, Steve
dc.contributor.authorShirran, Sally Lorna
dc.contributor.authorBotting, Catherine Helen
dc.contributor.authorBailey, Nathan William
dc.date.accessioned2017-04-17T23:33:43Z
dc.date.available2017-04-17T23:33:43Z
dc.date.issued2016-06
dc.identifier.citationPascoal , S C M , Liu , X , Ly , T , Fang , Y , Rockliffe , N , Paterson , S , Shirran , S L , Botting , C H & Bailey , N W 2016 , ' Rapid evolution and gene expression : a rapidly-evolving Mendelian trait that silences field crickets has widespread effects on mRNA and protein expression ' , Journal of Evolutionary Biology , vol. 29 , no. 6 , pp. 1234-1246 . https://doi.org/10.1111/jeb.12865en
dc.identifier.issn1420-9101
dc.identifier.otherPURE: 241919646
dc.identifier.otherPURE UUID: eb37b243-12ed-414f-b76b-84519e3c8296
dc.identifier.otherBibtex: urn:86afa2e396d25a6c1db7a302471da668
dc.identifier.otherPubMed: 26999731
dc.identifier.otherScopus: 85028235001
dc.identifier.otherORCID: /0000-0003-3516-3507/work/32169105
dc.identifier.otherWOS: 000382498900012
dc.identifier.otherORCID: /0000-0003-3531-7756/work/60888393
dc.identifier.urihttp://hdl.handle.net/10023/10624
dc.description.abstractA major advance in modern evolutionary biology is the ability to start linking phenotypic evolution in the wild with genomic changes that underlie that evolution. We capitalised on a rapidly-evolving Hawaiian population of crickets (Teleogryllus oceanicus) to test hypotheses about the genomic consequences of a recent Mendelian mutation of large effect which disrupts the development of sound-producing structures on male forewings. The resulting silent phenotype, flatwing, persists because of natural selection imposed by an acoustically-orienting parasitoid, but it interferes with mate attraction. We examined gene expression differences in developing wing buds of wild-type and flatwing male crickets using RNA-seq and quantitative proteomics. Most differentially expressed (DE) transcripts were down-regulated in flatwing males (625 up vs. 1716 down), whereas up and down-regulated proteins were equally represented (30 up and 34 down). Differences between morphs were clearly not restricted to a single pathway, and we recovered annotations associated with a broad array of functions that would not be predicted a priori. Using a candidate gene detection test based on homology we identified 30% of putative Drosophila wing development genes in the cricket transcriptome, but only 10% were DE. In addition to wing related annotations, endocrine pathways and several biological processes such as reproduction, immunity and locomotion were DE in the mutant crickets at both biological levels. Our results illuminate the breadth of genetic pathways that are potentially affected in the early stages of adaptation.
dc.format.extent13
dc.language.isoeng
dc.relation.ispartofJournal of Evolutionary Biologyen
dc.rights© 2016 European Society For Evolutionary Biology. Journal of Evolutionary Biology © 2016 European Society For Evolutionary Biology. This work is made available online in accordance with the publisher’s policies. This is the author created, accepted version manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at onlinelibrary.wiley.com / https://dx.doi.org/10.1111/jeb.12865en
dc.subjectRapid evolutionen
dc.subjectWing mutationen
dc.subjectTeleogryllus oceanicusen
dc.subjectTranscriptomeen
dc.subjectProteomeen
dc.subjectQH301 Biologyen
dc.subjectQH426 Geneticsen
dc.subject.lccQH301en
dc.subject.lccQH426en
dc.titleRapid evolution and gene expression : a rapidly-evolving Mendelian trait that silences field crickets has widespread effects on mRNA and protein expressionen
dc.typeJournal articleen
dc.contributor.sponsorNERCen
dc.contributor.sponsorNERCen
dc.contributor.sponsorNERCen
dc.contributor.sponsorThe Wellcome Trusten
dc.description.versionPostprinten
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews. School of Chemistryen
dc.contributor.institutionUniversity of St Andrews. EaSTCHEMen
dc.contributor.institutionUniversity of St Andrews. Centre for Biological Diversityen
dc.identifier.doihttps://doi.org/10.1111/jeb.12865
dc.description.statusPeer revieweden
dc.date.embargoedUntil2017-04-17
dc.identifier.grantnumberNe/I027800/1en
dc.identifier.grantnumberNE/G014906/1en
dc.identifier.grantnumberNE/L011255/1en
dc.identifier.grantnumber094476/Z/10/Zen


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