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dc.contributor.authorBossé, Janine T.
dc.contributor.authorLi, Yanwen
dc.contributor.authorRogers, Jon
dc.contributor.authorCrespo, Roberto Fernandez
dc.contributor.authorLi, Yinghui
dc.contributor.authorChaudhuri, Roy R.
dc.contributor.authorHolden, Matthew T. G.
dc.contributor.authorMaskell, Duncan J.
dc.contributor.authorTucker, Alexander W.
dc.contributor.authorWren, Brendan W.
dc.contributor.authorRycroft, Andrew N.
dc.contributor.authorLangford, Paul R.
dc.contributor.authorBRaDP1T Consortium
dc.identifier.citationBossé , J T , Li , Y , Rogers , J , Crespo , R F , Li , Y , Chaudhuri , R R , Holden , M T G , Maskell , D J , Tucker , A W , Wren , B W , Rycroft , A N , Langford , P R & BRaDP1T Consortium 2017 , ' Whole genome sequencing for surveillance of antimicrobial resistance in Actinobacillus pleuropneumoniae ' , Frontiers in Microbiology , vol. 8 , 311 .
dc.identifier.otherPURE: 249476823
dc.identifier.otherPURE UUID: 0b6c5b6f-c0e0-4012-a8f6-94e55ad6263e
dc.identifier.otherWOS: 000395371300001
dc.identifier.otherScopus: 85016568180
dc.identifier.otherORCID: /0000-0002-4958-2166/work/60196505
dc.identifier.otherWOS: 000395371300001
dc.descriptionThis work was supported by a Longer and Larger (LoLa) grant from the Biotechnology and Biological Sciences Research Council (BBSRC grant numbers BB/G020744/1, BB/G019177/1, BB/G019274/1, and BB/G018553/1), the UK Department for Environment, Food and Rural Affairs, and Zoetis (formerly Pfizer Animal Health) awarded to the Bacterial Respiratory Diseases of Pigs-1 Technology (BRaDP1T) consortium. MH was supported by the Wellcome Trust (grant number 098051). JR was funded from the former AHVLA’s Research and Development Internal Investment Fund (grant number RD0030c).en
dc.description.abstractThe aim of this study was to evaluate the correlation between antimicrobial resistance (AMR) profiles of 96 clinical isolates of Actinobacillus pleuropneumoniae, an important porcine respiratory pathogen, and the identification of AMR genes in whole genome sequence (wgs) data. Susceptibility of the isolates to nine antimicrobial agents (ampicillin, enrofloxacin, erythromycin, florfenicol, sulfisoxazole, tetracycline, tilmicosin, trimethoprim, and tylosin) was determined by agar dilution susceptibility test. Except for the macrolides tested, elevated MICs were highly correlated to the presence of AMR genes identified in wgs data using ResFinder or BLASTn. Of the isolates tested, 57% were resistant to tetracycline [MIC ≥ 4 mg/L; 94.8% with either tet(B) or tet(H)]; 48% to sulfisoxazole (MIC ≥ 256 mg/L or DD = 6; 100% with sul2), 20% to ampicillin (MIC ≥ 4 mg/L; 100% with blaROB-1), 17% to trimethoprim (MIC ≥ 32 mg/L; 100% with dfrA14), and 6% to enrofloxacin (MIC ≥ 0.25 mg/L; 100% with GyrAS83F). Only 33% of the isolates did not have detectable AMR genes, and were sensitive by MICs for the antimicrobial agents tested. Although 23 isolates had MIC ≥ 32 mg/L for tylosin, all isolates had MIC ≥ 32 mg/L for tylosin, all isolates had MIC ≤ 16 mg/L for both erythromycin and tilmicosin, and no macrolide resistance genes or known point mutations were detected. Other than the GyrAS83F mutation, the AMR genes detected were mapped to potential plasmids. In addition to presence on plasmid(s), the tet(B) gene was also found chromosomally either as part of a 56 kb integrative conjugative element (ICEApl1) in 21, or as part of a Tn7 insertion in 15 isolates. Our results indicate that, with the exception of macrolides, wgs data can be used to accurately predict resistance of A. pleuropneumoniae to the tested antimicrobial agents and provides added value for routine surveillance.
dc.relation.ispartofFrontiers in Microbiologyen
dc.rights© 2017 Bossé, Li, Rogers, Fernandez Crespo, Li, Chaudhuri, Holden, Maskell, Tucker, Wren, Rycroft, and Langford on behalf of the BRaDP1T Consortium. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.subjectAnimal infectionsen
dc.subjectAntimicrobial resistance genesen
dc.subjectIntegrative conjugative elementsen
dc.subjectRespiratory tracten
dc.subjectQR Microbiologyen
dc.subjectQH301 Biologyen
dc.titleWhole genome sequencing for surveillance of antimicrobial resistance in Actinobacillus pleuropneumoniaeen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Medicineen
dc.contributor.institutionUniversity of St Andrews. Infection Groupen
dc.contributor.institutionUniversity of St Andrews. Infection and Global Health Divisionen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.description.statusPeer revieweden

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