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dc.contributor.authorHuang, Xunbing
dc.contributor.authorWu, Huihui
dc.contributor.authorMcNeill, Mark Richard
dc.contributor.authorQin, Xinghu
dc.contributor.authorMa, Jingchuan
dc.contributor.authorTu, Xiongbing
dc.contributor.authorCao, Guangchun
dc.contributor.authorWang, Guangjun
dc.contributor.authorNong, Xiangqun
dc.contributor.authorZhang, Zehua
dc.date.accessioned2017-02-27T10:30:14Z
dc.date.available2017-02-27T10:30:14Z
dc.date.issued2016-08-26
dc.identifier.citationHuang , X , Wu , H , McNeill , M R , Qin , X , Ma , J , Tu , X , Cao , G , Wang , G , Nong , X & Zhang , Z 2016 , ' Quantitative analysis of diet structure by real-time PCR, reveals different feeding patterns by two dominant grasshopper species ' , Scientific Reports , vol. 6 , 32166 . https://doi.org/10.1038/srep32166en
dc.identifier.issn2045-2322
dc.identifier.otherPURE: 249230310
dc.identifier.otherPURE UUID: 453f1e9a-4b68-42e7-887b-1ecec2b1a1d4
dc.identifier.otherScopus: 84983652679
dc.identifier.otherORCID: /0000-0003-2351-3610/work/60631059
dc.identifier.urihttps://hdl.handle.net/10023/10372
dc.descriptionThis study was supported by the National Natural Science Foundation of China, 31471823, the Earmarked Fund for China Agriculture Research System, CARS-35-07, and the Innovation Project of Chinese Academy of Agricultural Science.en
dc.description.abstractStudies on grasshopper diets have historically employed a range of methodologies, each with certain advantages and disadvantages. For example, some methodologies are qualitative instead of quantitative. Others require long experimental periods or examine population-level effects, only. In this study, we used real-time PCR to examine diets of individual grasshoppers. The method has the advantage of being both fast and quantitative. Using two grasshopper species, Oedaleus asiaticus and Dasyhippus barbipes, we designed ITS primer sequences for their three main host plants, Stipa krylovii, Leymus chinensis and Cleistogenes squarrosa and used real-time PCR method to test diet structure both qualitatively and quantitatively. The lowest detection efficiency of the three grass species was ~80% with a strong correlation between actual and PCR-measured food intake. We found that Oedaleus asiaticus maintained an unchanged diet structure across grasslands with different grass communities. By comparison, Dasyhippus barbipes changed its diet structure. These results revealed why O. asiaticus distribution is mainly confined to Stipa-dominated grassland, and D. barbipes is more widely distributed across Inner Mongolia. Overall, real-time PCR was shown to be a useful tool for investigating grasshopper diets, which in turn offers some insight into grasshopper distributions and improved pest management.
dc.format.extent11
dc.language.isoeng
dc.relation.ispartofScientific Reportsen
dc.rights© The Author(s) 2016. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/en
dc.subjectQH301 Biologyen
dc.subjectNDASen
dc.subject.lccQH301en
dc.titleQuantitative analysis of diet structure by real-time PCR, reveals different feeding patterns by two dominant grasshopper speciesen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.identifier.doihttps://doi.org/10.1038/srep32166
dc.description.statusPeer revieweden


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