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dc.contributor.authorGotz, Dorothee
dc.contributor.authorPaytubi, Sonia
dc.contributor.authorMunro, Stacey
dc.contributor.authorLundgren, Magnus
dc.contributor.authorBernander, Rolf
dc.contributor.authorWhite, Malcolm F.
dc.date.accessioned2010-10-14T13:05:07Z
dc.date.available2010-10-14T13:05:07Z
dc.date.issued2007
dc.identifier.citationGotz , D , Paytubi , S , Munro , S , Lundgren , M , Bernander , R & White , M F 2007 , ' Responses of hyperthermophilic crenarchaea to UV irradiation ' , Genome Biology , vol. 8 , no. 10 , pp. R220 . https://doi.org/10.1186/gb-2007-8-10-r220en
dc.identifier.issn1465-6906
dc.identifier.otherPURE: 1657070
dc.identifier.otherPURE UUID: 5fd8d273-825c-4529-9050-61a68d9545cb
dc.identifier.otherWOS: 000252100900018
dc.identifier.otherScopus: 36749072168
dc.identifier.otherORCID: /0000-0003-1543-9342/work/47136078
dc.identifier.urihttp://hdl.handle.net/10023/1029
dc.descriptionThis work was supported by a dedicated functional genomics grant from the Swedish Research Council and the Swedish Graduate Research School in Genomics and Bioinformatics to RB, and by grants from the Wellcome Trust and BBSRC to MFW.en
dc.description.abstractBackground: DNA damage leads to cellular responses that include the increased expression of DNA repair genes, repression of DNA replication and alterations in cellular metabolism. Archaeal information processing pathways resemble those in eukaryotes, but archaeal damage response pathways remain poorly understood. Results: We analyzed the transcriptional response to UV irradiation in two related crenarchaea, Sulfolobus solfataricus and Sulfolobus acidocaldarius. Sulfolobus species encounter high levels of DNA damage in nature, as they inhabit high temperature, aerobic environments and are exposed to sunlight. No increase in expression of DNA repair genes following UV irradiation was observed. There was, however, a clear transcriptional response, including repression of DNA replication and chromatin proteins. Differential effects on the expression of the three transcription factor B ( tfb) genes hint at a mechanism for the modulation of transcriptional patterns in response to DNA damage. TFB3, which is strongly induced following UV irradiation, competes with TFB1 for binding to RNA polymerase in vitro, and may act as a repressor of transcription or an alternative transcription factor for certain promoters. Conclusion: A clear response to DNA damage was observed, with down-regulation of the DNA replication machinery, changes in transcriptional regulatory proteins, and up-regulation of the biosynthetic enzymes for beta-carotene, which has UV protective properties, and proteins that detoxify reactive oxygen species. However, unlike eukaryotes and bacteria, there was no induction of DNA repair proteins in response to DNA damage, probably because these are expressed constitutively to deal with increased damage arising due to high growth temperatures.
dc.format.extent18
dc.language.isoeng
dc.relation.ispartofGenome Biologyen
dc.rightsThis is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.subjectArchaeon sulfolobus-solfataricusen
dc.subjectDNA-binding proteinen
dc.subjectCell-cycleen
dc.subjectFactor requirementsen
dc.subjectRNA-polymeraseen
dc.subjectRepairen
dc.subjectDamageen
dc.subjectTranscriptionen
dc.subjectGenomeen
dc.subjectAcidocaldariusen
dc.subjectQH426 Geneticsen
dc.subject.lccQH426en
dc.titleResponses of hyperthermophilic crenarchaea to UV irradiationen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1186/gb-2007-8-10-r220
dc.description.statusPeer revieweden
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=36749072168&partnerID=8YFLogxKen


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