Show simple item record

Files in this item

Thumbnail

Item metadata

dc.contributor.authorShi, Xiaohong
dc.contributor.authorBotting, Catherine Helen
dc.contributor.authorLi, Ping
dc.contributor.authorNiglas, Mark
dc.contributor.authorBrennan, Benjamin
dc.contributor.authorShirran, Sally Lorna
dc.contributor.authorSzemiel, Agnieszka Marta
dc.contributor.authorElliott, Richard Michael
dc.date.accessioned2017-01-21T00:32:27Z
dc.date.available2017-01-21T00:32:27Z
dc.date.issued2016-08-02
dc.identifier244781085
dc.identifieraa25ae23-fdf9-4f37-b35f-8a7a3e419da2
dc.identifier84982683066
dc.identifier000380586600072
dc.identifier.citationShi , X , Botting , C H , Li , P , Niglas , M , Brennan , B , Shirran , S L , Szemiel , A M & Elliott , R M 2016 , ' Bunyamwera orthobunyavirus glycoprotein precursor is processed by cellular signal peptidase and signal peptide peptidase ' , Proceedings of the National Academy of Sciences of the United States of America , vol. 113 , no. 31 , pp. 8825-8830 . https://doi.org/10.1073/pnas.1603364113en
dc.identifier.issn1091-6490
dc.identifier.otherORCID: /0000-0003-3516-3507/work/32169104
dc.identifier.urihttps://hdl.handle.net/10023/10142
dc.descriptionThis study was supported by Wellcome Trust Grant 099220/B/12/Z (to R.M.E.) and Grant 094476/Z/10/Z that funded the purchase of the TripleTOF 5600 mass spectrometer at the Biomedical Sciences Research Complex (BSRC) of University of St. Andrews.en
dc.description.abstractBunyamwera virus (BUNV) is the prototype of the Orthobunyavirus genus and Bunyaviridae family that contains important human and animal pathogens. The cleavage mechanism of orthobunyavirus glycoprotein precursor (GPC) and the host proteases involved have not been clarified. Here we found that NSm and Gc contain their own internal signal peptides, which mediate the GPC cleavage by host signal peptidase and signal peptide peptidase (SPP). Furthermore, the NSm domain-I plays an important postcleavage role in cell fusion. Our data clarified the implication of host proteases in the processing of the orthobunyavirus GPC. This work identifies SPP as a potential intervention target, and the knowledge we gained will benefit preventive strategies against other orthobunyavirus infections.
dc.format.extent6
dc.format.extent865325
dc.language.isoeng
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of Americaen
dc.subjectBunyaviruaen
dc.subjectBunyamwera virusen
dc.subjectGlycoprotein precursor processingen
dc.subjectSignal peptidaseen
dc.subjectSignal peptide peptidaseen
dc.subjectQD Chemistryen
dc.subjectQH301 Biologyen
dc.subjectBDCen
dc.subjectR2Cen
dc.subject.lccQDen
dc.subject.lccQH301en
dc.titleBunyamwera orthobunyavirus glycoprotein precursor is processed by cellular signal peptidase and signal peptide peptidaseen
dc.typeJournal articleen
dc.contributor.sponsorThe Wellcome Trusten
dc.contributor.institutionUniversity of St Andrews. School of Chemistryen
dc.contributor.institutionUniversity of St Andrews. EaSTCHEMen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.identifier.doi10.1073/pnas.1603364113
dc.description.statusPeer revieweden
dc.identifier.grantnumber094476/Z/10/Zen


This item appears in the following Collection(s)

Show simple item record