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Lasing in live mitotic and non-phagocytic cells by efficient delivery of microresonators
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dc.contributor.author | Schubert, Marcel | |
dc.contributor.author | Volckaert, Klara | |
dc.contributor.author | Karl, Markus | |
dc.contributor.author | Morton, Andrew | |
dc.contributor.author | Liehm, Philipp | |
dc.contributor.author | Miles, Gareth Brian | |
dc.contributor.author | Powis, Simon John | |
dc.contributor.author | Gather, Malte Christian | |
dc.date.accessioned | 2017-01-20T00:32:38Z | |
dc.date.available | 2017-01-20T00:32:38Z | |
dc.date.issued | 2017-01-19 | |
dc.identifier.citation | Schubert , M , Volckaert , K , Karl , M , Morton , A , Liehm , P , Miles , G B , Powis , S J & Gather , M C 2017 , ' Lasing in live mitotic and non-phagocytic cells by efficient delivery of microresonators ' , Scientific Reports , vol. 7 , 40877 . https://doi.org/10.1038/srep40877 | en |
dc.identifier.issn | 2045-2322 | |
dc.identifier.other | PURE: 247319635 | |
dc.identifier.other | PURE UUID: 8a73d3d6-456c-47d6-b39b-8c7a2965d0c9 | |
dc.identifier.other | Scopus: 85010332910 | |
dc.identifier.other | ORCID: /0000-0002-8624-4625/work/29642124 | |
dc.identifier.other | ORCID: /0000-0002-4857-5562/work/47136444 | |
dc.identifier.other | ORCID: /0000-0002-8739-4852/work/36937593 | |
dc.identifier.other | ORCID: /0000-0003-4218-2984/work/60195318 | |
dc.identifier.other | WOS: 000392225000001 | |
dc.identifier.uri | https://hdl.handle.net/10023/10132 | |
dc.description | We acknowledge support from the ERC Starting Grant ABLASE (640012), the Scottish Funding Council (via SUPA), and the European Union Marie Curie Career Integration Grant (PCIG12-GA-2012-334407). MS acknowledges funding by the European Commission through a Marie Sklodowska-Curie Individual Fellowship (659213). MK and PL acknowledge funding from the EPSRC DTG (EP/M506631/1 and EP/L505079/1). | en |
dc.description.abstract | Reliable methods to individually track large numbers of cells in real time are urgently needed to advance our understanding of important biological processes like cancer metastasis, neuronal network development and wound healing. It has recently been suggested to introduce microscopic whispering gallery mode lasers into the cytoplasm of cells and to use their characteristic, size dependent emission spectrum as optical barcode but so far there is no evidence that this approach is generally applicable. Here, we describe a method that drastically improves intracellular delivery of resonators for several cell types, including mitotic and non-phagocytic cells. In addition, we characterize the influence of resonator size on the spectral characteristics of the emitted laser light and identify an optimum size range that facilitates tagging and tracking of thousands of cells simultaneously. Finally, we observe that the microresonators remain internalized by cells during cell division, which enables tagging several generations of cells. | |
dc.language.iso | eng | |
dc.relation.ispartof | Scientific Reports | en |
dc.rights | Copyright the Authors 2017. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ | en |
dc.subject | QC Physics | en |
dc.subject | RC0254 Neoplasms. Tumors. Oncology (including Cancer) | en |
dc.subject | RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry | en |
dc.subject | DAS | en |
dc.subject | SDG 3 - Good Health and Well-being | en |
dc.subject.lcc | QC | en |
dc.subject.lcc | RC0254 | en |
dc.subject.lcc | RC0321 | en |
dc.title | Lasing in live mitotic and non-phagocytic cells by efficient delivery of microresonators | en |
dc.type | Journal article | en |
dc.contributor.sponsor | European Research Council | en |
dc.contributor.sponsor | European Commission | en |
dc.contributor.sponsor | European Commission | en |
dc.description.version | Publisher PDF | en |
dc.contributor.institution | University of St Andrews. School of Physics and Astronomy | en |
dc.contributor.institution | University of St Andrews. School of Psychology and Neuroscience | en |
dc.contributor.institution | University of St Andrews. Institute of Behavioural and Neural Sciences | en |
dc.contributor.institution | University of St Andrews. School of Medicine | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.contributor.institution | University of St Andrews. Cellular Medicine Division | en |
dc.identifier.doi | https://doi.org/10.1038/srep40877 | |
dc.description.status | Peer reviewed | en |
dc.date.embargoedUntil | 2017-01-19 | |
dc.identifier.grantnumber | 640012 | en |
dc.identifier.grantnumber | PCIG12-GA-2012-334407 | en |
dc.identifier.grantnumber | 659213 | en |
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