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dc.contributor.authorSchubert, Marcel
dc.contributor.authorVolckaert, Klara
dc.contributor.authorKarl, Markus
dc.contributor.authorMorton, Andrew
dc.contributor.authorLiehm, Philipp
dc.contributor.authorMiles, Gareth Brian
dc.contributor.authorPowis, Simon John
dc.contributor.authorGather, Malte Christian
dc.date.accessioned2017-01-20T00:32:38Z
dc.date.available2017-01-20T00:32:38Z
dc.date.issued2017-01-19
dc.identifier.citationSchubert , M , Volckaert , K , Karl , M , Morton , A , Liehm , P , Miles , G B , Powis , S J & Gather , M C 2017 , ' Lasing in live mitotic and non-phagocytic cells by efficient delivery of microresonators ' , Scientific Reports , vol. 7 , 40877 . https://doi.org/10.1038/srep40877en
dc.identifier.issn2045-2322
dc.identifier.otherPURE: 247319635
dc.identifier.otherPURE UUID: 8a73d3d6-456c-47d6-b39b-8c7a2965d0c9
dc.identifier.otherScopus: 85010332910
dc.identifier.otherORCID: /0000-0002-8624-4625/work/29642124
dc.identifier.otherORCID: /0000-0002-4857-5562/work/47136444
dc.identifier.otherORCID: /0000-0002-8739-4852/work/36937593
dc.identifier.otherORCID: /0000-0003-4218-2984/work/60195318
dc.identifier.otherWOS: 000392225000001
dc.identifier.urihttps://hdl.handle.net/10023/10132
dc.descriptionWe acknowledge support from the ERC Starting Grant ABLASE (640012), the Scottish Funding Council (via SUPA), and the European Union Marie Curie Career Integration Grant (PCIG12-GA-2012-334407). MS acknowledges funding by the European Commission through a Marie Sklodowska-Curie Individual Fellowship (659213). MK and PL acknowledge funding from the EPSRC DTG (EP/M506631/1 and EP/L505079/1).en
dc.description.abstractReliable methods to individually track large numbers of cells in real time are urgently needed to advance our understanding of important biological processes like cancer metastasis, neuronal network development and wound healing. It has recently been suggested to introduce microscopic whispering gallery mode lasers into the cytoplasm of cells and to use their characteristic, size dependent emission spectrum as optical barcode but so far there is no evidence that this approach is generally applicable. Here, we describe a method that drastically improves intracellular delivery of resonators for several cell types, including mitotic and non-phagocytic cells. In addition, we characterize the influence of resonator size on the spectral characteristics of the emitted laser light and identify an optimum size range that facilitates tagging and tracking of thousands of cells simultaneously. Finally, we observe that the microresonators remain internalized by cells during cell division, which enables tagging several generations of cells.
dc.language.isoeng
dc.relation.ispartofScientific Reportsen
dc.rightsCopyright the Authors 2017. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/en
dc.subjectQC Physicsen
dc.subjectRC0254 Neoplasms. Tumors. Oncology (including Cancer)en
dc.subjectRC0321 Neuroscience. Biological psychiatry. Neuropsychiatryen
dc.subjectDASen
dc.subjectSDG 3 - Good Health and Well-beingen
dc.subject.lccQCen
dc.subject.lccRC0254en
dc.subject.lccRC0321en
dc.titleLasing in live mitotic and non-phagocytic cells by efficient delivery of microresonatorsen
dc.typeJournal articleen
dc.contributor.sponsorEuropean Research Councilen
dc.contributor.sponsorEuropean Commissionen
dc.contributor.sponsorEuropean Commissionen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Physics and Astronomyen
dc.contributor.institutionUniversity of St Andrews. School of Psychology and Neuroscienceen
dc.contributor.institutionUniversity of St Andrews. Institute of Behavioural and Neural Sciencesen
dc.contributor.institutionUniversity of St Andrews. School of Medicineen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews. Cellular Medicine Divisionen
dc.identifier.doihttps://doi.org/10.1038/srep40877
dc.description.statusPeer revieweden
dc.date.embargoedUntil2017-01-19
dc.identifier.grantnumber640012en
dc.identifier.grantnumberPCIG12-GA-2012-334407en
dc.identifier.grantnumber659213en


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