Files in this item
A study of the behaviour and interactions of the novel FERM protein Willin
Item metadata
dc.contributor.advisor | Gunn-Moore, Frank J. | |
dc.contributor.advisor | Guild, Simon | |
dc.contributor.author | Herron, Lissa Rocha | |
dc.coverage.spatial | 203 | en |
dc.date.accessioned | 2008-02-04T10:50:09Z | |
dc.date.available | 2008-02-04T10:50:09Z | |
dc.date.issued | 2008 | |
dc.identifier | uk.bl.ethos.552079 | |
dc.identifier.uri | https://hdl.handle.net/10023/418 | |
dc.description.abstract | Willin is a novel member of the Four-point-one Ezrin Radixin Moesin (FERM) protein superfamily, containing an N-terminal FERM domain most like the Ezrin-Radixin-Moesin (ERM) family but also the closely related protein Merlin. Willin was initially discovered as a yeast two-hybrid binding partner of neurofascin155, and this interaction has now been confirmed by both co-localisation studies and the use of two different biochemical methods. Like neurofascin155, Willin also localises to detergent resistant membranes, and like the ERM family, it is able to bind to phospholipids. The expression of Willin appears to be toxic as the production of cell-lines stably expressing Willin proved to be not possible and this appears to be because it induces apoptosis in cultured cells. This is a proliferation control function consistent with the suggestion that Willin is the human homologue of the Drosophila tumour suppressor ‘Expanded’. Three antibodies to Willin were also characterised and a novel splice variant, Willin2, subcloned into a GFP-tagged plasmid for comparison with the original form. | en |
dc.format.extent | 4561610 bytes | |
dc.format.mimetype | application/pdf | |
dc.language.iso | en | en |
dc.publisher | University of St Andrews | |
dc.rights | Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported | |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/ | |
dc.subject | Willin | en |
dc.subject | FERM | en |
dc.subject | Merlin | en |
dc.subject | Ezrin | en |
dc.subject | Radixin | en |
dc.subject | Moesin | en |
dc.subject | Neurofascin | en |
dc.subject | L1 | en |
dc.subject.lcc | QP552.C96H4 | |
dc.subject.lcsh | Cytoskeletal proteins | en |
dc.subject.lcsh | Gene expression | en |
dc.title | A study of the behaviour and interactions of the novel FERM protein Willin | en |
dc.type | Thesis | en |
dc.type.qualificationlevel | Doctoral | en |
dc.type.qualificationname | PhD Doctor of Philosophy | en |
dc.publisher.institution | The University of St Andrews | en |
This item appears in the following Collection(s)
Except where otherwise noted within the work, this item's licence for re-use is described as Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported
Items in the St Andrews Research Repository are protected by copyright, with all rights reserved, unless otherwise indicated.