Show simple item record

Files in this item


Item metadata

dc.contributor.advisorJones, John T.
dc.contributor.advisorBlok, Vivian
dc.contributor.advisorBryan, Glenn J.
dc.contributor.authorGartner, Ulrike
dc.description.abstractThe potato cyst nematodes (PCN) Globodera pallida and G. rostochiensis are economically important potato pests in almost all regions where potato is grown. Studying the composition, distribution and virulence of PCN populations in fields, and finding new sources of naturally occurring resistance in wild potato species is important for the management of these pests. In Scotland, up to three different introductions of G. pallida, determined by mitotyping, were found to be present in fields. To investigate whether cysts in a population show a correlation between mitotype and different virulence levels, “single cyst” lines were generated, mitotyped, and their virulence to different potato cultivars was determined. One mitotype was shown to correlate with G. pallida pathotype Pa3, but overall, the mitotypes are not usable as reliable virulence markers. A phylogenetic analysis was performed to determine the relationships between British G. pallida populations. In addition, single nucleotide polymorphisms (SNPs) in genomic DNA were identified that represent candidate virulence markers. A screen of wild potato germplasm was undertaken to identify new resistance against G. pallida. The diploid species Solanum spegazzinii Bitter accession 7195 shows resistance to G. pallida pathotypes Pa1 and Pa2/3. A cross and first backcross of S. spegazzinii with S. tuberosum group Phureja cultivar Mayan Gold was performed, and the level of resistance to G. pallida Pa2/3 was determined in progeny. Bulked-segregant analysis sing generic mapping enrichment sequencing and genotyping-by-sequencing was performed to identify SNPs that are genetically linked to the resistance, using S. tuberosum group Phureja clone DM1-3 516 R44 as a reference genome. These SNPs were converted into allele specific PCR assays, and the resistance was mapped using graphical genotyping. The resistance was successfully introgressed into a tetraploid potato cultivar by a tetraploid-diploid interploidy cross.en_US
dc.subjectPotato cyst nematodes (PCN)en_US
dc.subjectGlobodera pallidaen_US
dc.subjectKompetitive allele specific PCR (KASP) assaysen_US
dc.subjectIntrogressing resistance using interploidy breedingen_US
dc.subjectPhylogeny of British G. pallida populationsen_US
dc.subjectPathotype markeren_US
dc.subject.lcshPotatoes--Disease and pest resistanceen
dc.subject.lcshGoldern nematodeen
dc.subject.lcshGlobodera pallidaen
dc.titleExploring and exploiting the resistance to Globodera pallida in potatoen_US
dc.contributor.sponsorNational Institute of Food and Agriculture (USDA NIFA). Globodera alliance projecten_US
dc.type.qualificationnamePhD Doctor of Philosophyen_US
dc.publisher.institutionThe University of St Andrewsen_US
dc.publisher.departmentThe James Hutton Instituteen_US

This item appears in the following Collection(s)

Show simple item record