Defining a role for the peduncolopontine tegmental nucleus in striatal outflow
Abstract
The pedunculopontine tegmental nucleus (PPTg) lies within the pontomesencephalon
and contains cholinergic and non-cholinergic neurones. It has extensive afferent and
efferent connections throughout the brain. Early research suggested a role for the
PPTg in the mediation of locomotor activity, and it was believed to form the major
substrate of the electrophysiologically identified mesencephalic locomotor region
(rviLR). Studies using selective excitotoxic lesions of the PPTg demonstrated that it
has no role in the mediation of spontaneous or nucleus accumbens-induced (NAcc)
locomotion. However evidence has suggested that the cuneiform nucleus (CNF) and
not the PPTg is the main locus of the .MLR. The effects of bilateral ibotenate CNF
lesions on spontaneous and amphetamine-induced locomotion stimulated from the
NAcc were therefore investigated. CNF lesions had no effect on either type of
locomotor activity.
Bilateral ibotenate lesions of the PPTg have been shown to influence the
expression of orofacial stereotypies following administration of systemic
amphetamine. Oral stereotypies can be elicited reliably by direct stimulation of the
ventrolateral caudate-putamen (VLCP). This thesis sought to clarify the role of the
PPTg in the mediation of oral stereotypies, by combining bilateral ibotenate lesions of
the PPTg with direct microinjection of amphetamine into the VLCP. Lesions of the
PPTg caused a shift in the dose response curve to amphetamine resulting in an
increase in the incidence and intensity of oro facial stereotypies at lower doses. Thus
the PPTg appears to have inhibitory control over the expression of orofacial
behaviors.
It is hypothesised that while neither the PPTg nor the CNF have a role in the
mediation of locomotor activity per se they may provide an integrative functional
role, which influences motor outflow. The role of the CNF in the transmission of
nociception and a role for the PPTg in the mediation of striatal outflow is discussed.
Type
Thesis, PhD Doctor of Philosophy
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