Show simple item record

Files in this item

Thumbnail

Item metadata

dc.contributor.advisorRyan, Martin Denisen
dc.contributor.authorKinver, Andreaen
dc.coverage.spatial88 pen
dc.date.accessioned2021-04-08T08:57:16Z
dc.date.available2021-04-08T08:57:16Z
dc.date.issued2003
dc.identifier.urihttps://hdl.handle.net/10023/21861
dc.description.abstractPicornavirus-infected cells are characterised by the appearance of smooth vesicles and the disappearance of the Golgi apparatus. Replication of the viral RNA takes place on these virus-induced vesicles. Expression of the viral protein 2BC, and to some extent 2C, has similar effects on cells. Protein 2C has been proposed to hold these replication complexes together and is known to bind to the membranes, however the mechanism of this binding is unknown. Previous work by Knox et al showed an interaction between FMDV protein 2C and the protein kinase A anchoring protein 10 (AKAP 10). The 2C gene from the CA9, ECHO 22, FMDV, HAV HM175, HAV wt1, HeCV, HRV 85 and TME picornaviruses were cloned into both translation and expression vectors (the FMDV and HRV 85 were donated by C. Knox and N. Moir respectively). Translation products were then used in a binding assay and interaction with AKAP 10 was shown for ECHO 22, FMDV, HAV wt1, HeCV, HRV 85 and TME. These results indicate AKAP 10 binding as a general function of 2C in all picornaviruses. An expression system was optimised for HRV 85 2ABC, 2BC and 2C proteins, and was shown to produce the other picornavirus 2C proteins at similar levels. Cellular expression of protein 2BC is known to bring about membrane rearrangements and the binding of 2C to AKAP 10 may help explain this aspect of the cytopathic effect and the targeting of virus replication to membranes.en
dc.language.isoenen
dc.publisherUniversity of St Andrewsen
dc.subject.lccQR410.K5
dc.subject.lcshPicornavirusesen
dc.titleCloning, expression and functional analysis of picornavirus protein 2Cen
dc.typeThesisen
dc.type.qualificationlevelDoctoralen
dc.type.qualificationnameMPhil Master of Philosophyen
dc.publisher.institutionThe University of St Andrewsen


This item appears in the following Collection(s)

Show simple item record