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dc.contributor.advisorField, Rob
dc.contributor.authorHarrison, Jennifer Amanda
dc.coverage.spatial156 p.en_US
dc.description.abstractThe protozoan blood-borne parasite Trypanosoma cruzi is the causative agent of Chagas' disease, an enervating and often fatal illness prevalent in South and Central America for which there is no effective treatment. T. cruzi has a cell-surface trans- sialidase which transfers sialic acid from mammalian oligosaccharides to the parasite. This action allows adhesion to and invasion of mammalian cells, subsequently allowing parasitic replication. This protein therefore is exploitable and represents a potential target for the development of chemotherapeutic agents. This thesis describes the purification of recombinant trans-sialidase and the development of a rapid, reliable spectrophotometric coupled assay to measure trans-sialidase activity. It also details the use of three mutually exclusive synthetic oligosaccharide libraries to map substrate recognition for the enzyme. Synthetic fragments of the natural branched oligosaccharide substrates have also been sialylated on a preparative scale, demonstrating the use of trans-sialidase in synthetic oligosaccharide chemistry.en_US
dc.publisherUniversity of St Andrews
dc.subject.lcshPlasma and serum
dc.subject.lcshPlasma and serumen
dc.subject.lcshPlasma and serumen
dc.titleInvestigation of the substrate specificity of recombinant Trypanosoma cruzi trans-sialidaseen_US
dc.type.qualificationnamePhD Doctor of Philosophyen_US
dc.publisher.institutionThe University of St Andrewsen_US

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