Actions of neuropeptides on mouse spinal neurones in culture
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1] Spinal cords from mouse embryos were successfully prepared and maintained in primary dissociated cell culture, for periods in excess of 10 weeks. 2] Stable intracellular recordings were made from spinal neurones which had been sustained in these cultures. 3] Experiments were made on these spinal neurones using various amino acids and peptides. Solutions of these compounds were discretely applied by pressure ejection. 4] L-Glutamate, GABA and glycine evoked responses which appeared the same as those documented previously. 5] Ethylene-diamine did not evoke a response from the spinal neurones tested. 6] Only a small percentage of the spinal neurones responded to met5- and leu5 - enkephalin, FMRFamide, neurotensin and glycyl L-glutamine. Supplementing the cultures with tissue from other organs did not increase the percentage of spinal neurones which were capable of responding to peptide. 7] Met5 -enkephalin and leu5 -enkephalin each evoked responses from the spinal neurones. 8] The enkephalin-evoked depolarizations accompanied by an increased input resistance were apparently voltage dependent. These responses were abolished at potentials more negative than -90mV and did not invert under normal recording conditions. 9] The enkephalin-evoked depolarizations associated with a decreased input resistance had extrapolated inversion potentials of -20mV. No voltage dependence was seen. 10] Enkephalins also evoked responses which had an inversion potential close to the resting membrane potential. These were accompanied by a decreased input resistance and were not desensitized by prolonged application of peptide. 11] None of these responses showed obvious desensitization with prolonged application, however, they were all attenuated by naloxone. 12] Met5 -enkephalin was apparently more potent than leu5 -enkephalin on a small number of neurones. Furthermore, met5 -enkephalin application, during the weaker response from those neurones to leu5 -enkephalin, evoked a attenuated response. 13] FMRFamide evoked two responses from these spinal neurones. These responses were seen separately and mixed. In the latter case they were referred to as biphasic responses. 14] The depolarizing response to FMRFamide was accompanied by an increase in input resistance. Potassium had some involvement in these responses. 15] The FMRFamide responses which were accompanied by a decreased input resistance showed a great variety of inversion potentials between neurones. These actions were dependent upon sodium and chloride ions. 16] Enkephalin and FMRFamide, when applied separately to the same spinal neurone, did not evoke the same response. 17] Responses evoked by neurotensin were hyperpolarizations associated with a decreased input resistance. These responses were dependent upon potassium and independent of chloride ions. 18] Glycyl L-glutamine evoked two types of hyperpolarizing response from the spinal neurones. These could appear separately or combined. 19] The faster responses to glycyl L-glutamine were apparently dependent on potassium ions. 20] The slower responses to glycyl L-glutamine were apparently insensitive to changes in extracellular potassium or chloride. However, these responses were sensitive to intracellular injection of chloride ions. 21] At concentrations of peptide which evoked a response from other spinal neurones, none of the peptides produced any measurable modulation of amino acid-evoked responses.
Thesis, PhD Doctor of Philosophy
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