Nylon tube immobilized enzymes in continuous-flow analysis
Abstract
The chemistry required for the immobilization of enzymes onto the inner surface of nylon tubing is demonstrated and conditions are optimised. Nylon tube immobilised enzymes are then introduced into continuous-flow systems so that their flow characteristics can be determined and the chemistry of immobilization best suited to continuous-flow analysis ascertained. The practical use of immobilized enzyme derivatives is evaluated in four separate methodologies end their stability and pH dependence is assessed.
Type
Thesis, PhD Doctor of Philosophy
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