Some studies on soluble and immobilised dehydrogenases

Abstract

1. YADH was immobilised on two aminoethylcelluloses and their properties were compared. 2. Experimental conditions for the coupling of YADH to Cellex-AE were optimised; these conditions were used for immobilising LDH and MDH on Cellex-AE. 3. The effect of sodium borohydride reduction on the stability of YADH, LDH and MDH immobilised on Cellex-AE was studied. 4. The effect of pH on the stability of soluble YADH, LDH and MDH was studied. The results were compared to those for the three enzymes immobilised on Cellex-AE. 5. The effect of temperature on soluble YADH, LDH and MDH was compared to the effect of temperature on several immobilised derivatives of these enzymes. 6. YADH was immobilised on DEAE-cellulose. The pH variation of the kinetic parameters of the immobilised derivatives were compared to those of soluble YADH. 7. LDH was immobilised on both Cellex-AE and PEI. The kinetic parameters and stabilities of these two derivatives were compared. 8. LDH was immobilised on NP/3 Nylon Powder. The pH variation of the kinetic-parameters of the immobilised derivatives were compared to those of soluble LDH. 9. The change in equilibrium constant when YADH and LDH were immobilised on macromolecular supports was studied. A theory was developed to explain these changes.