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Passive potassium fluxes in cultural HeLa and MDCK cells
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dc.contributor.advisor | Simmons, N. L. | |
dc.contributor.author | Tivey, David Robert | |
dc.coverage.spatial | 350 p. | en_US |
dc.date.accessioned | 2018-06-12T15:22:39Z | |
dc.date.available | 2018-06-12T15:22:39Z | |
dc.date.issued | 1986 | |
dc.identifier.uri | https://hdl.handle.net/10023/13972 | |
dc.description.abstract | The cultured cell lines HeLa (human cervical carcinoma) and MDCK (renal epithelial) possess a trans-membrane K+ transport that can be pharmacologically divided into ouabain-sensitive, diuretic-sensitive and ouabain- and diuretic-insensitive components. The K+ transport of primary interest in this thesis is that sensitive to "loop" diuretic. Analysis of the cation and anion dependencies and "loop" diuretic sensitivity of this K+ transport indicates that it is mediated via a Na+ K+ Cl- "cotransporter" as has already been reported in other cell types. Cell shrinkage stimulated the diuretic-sensitive K+ transport in both HeLa and MDCK cells. Analysis of the Na+ K+ and Cl- dependency of this K+ flux demonstrated that this stimulation is principally due to an increase in the maximal velocity (Vmax) of this K+ transport. This stimulated K+ flux was not regulated by cAMP nor was the response modified by elevated cellular cAMP or by low extracellular Ca2+ (+ EGTA) media. The increased maximal velocity for K+ transport cannot simply be considered to be an absolute increase in the number of Na+ K+ Cl- cotransport units in the membrane. However, a possible regulation of the Na+ K+ Cl- "cotransport" turnover rate (molecules/site. second) may be Involved. In this study the Na+ K+ Cl- "cotransport" of both HeLa and MDCK cells was inhibited in metabolically depleted cells (metabolic inhibition induced by the limited-metabolisable sugar 2 deoxy-D-glucose). This effect may be considered a secondary response of cell swelling however, since exposure of metabolically depleted cells to hyperosmolar media re-activates the Na+ K+ C1- "cotransport" in both HeLa and MDCK cells. | en_US |
dc.language.iso | en | en_US |
dc.publisher | University of St Andrews | |
dc.subject.lcc | QH601.P7T5 | en |
dc.subject.lcsh | Membranes (Biology) | en |
dc.title | Passive potassium fluxes in cultural HeLa and MDCK cells | en_US |
dc.type | Thesis | en_US |
dc.contributor.sponsor | Science and Engineering Research Council (SERC) | en_US |
dc.type.qualificationlevel | Doctoral | en_US |
dc.type.qualificationname | PhD Doctor of Philosophy | en_US |
dc.publisher.institution | The University of St Andrews | en_US |
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